Rabbit Anti-BUB1 Recombinant Antibody (clone R05-1E2) (CAT#: VS3-XY3281)
This antibody is a recombinant rabbit antibody targeting the BUB1 (BUB1 mitotic checkpoint serine/threonine kinase), which acts as a substrate for anaphase-promoting complex or cyclosome in complex with its activator CDH1. It reacts with human, mouse and rat BUB1. It has been reported for use in Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of western blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6123/967_C5_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6123/967_C5_4_selected.jpg
Figure 2 RNA cell line category: Cell line enhanced (HEL)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169679-BUB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169679-BUB1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BUB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-1E2
- Applications
- WB, IHC-P
- Related Disease
- Microcephaly 30, primary, autosomal recessive (MCPH30)
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- BUB1A; BUB1L; hBUB1; MCPH30
- Gene ID
- 699
- UniProt ID
- O43683
- Long Name
- BUB1 mitotic checkpoint serine/threonine kinase
- Cellular Localization
- Centromere, Chromosome, Kinetochore, Nucleus
- Post Translation Modifications
- Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.
- Protein Refseq
- XP_047301572.1
- Function
- Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Promotes the centromeric localization of TOP2A.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0944MZ | Mouse Anti-BUB1 Recombinant Antibody (clone CBLS-179) | IF | Mouse IgG1 |
| ZG-0574J | Rabbit Anti-BUB1 Recombinant Antibody (clone 9C11) | ELISA, WB | Rabbit IgG |
| VS1-YJ16 | Mouse Anti-BUB1 Recombinant Antibody (clone 14H5) | IF, WB, IP | Mouse IgG1 |
| VS7-HM274 | Mouse Anti-BUB1 Recombinant Antibody (clone 1A11C7) | FCM | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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