Human Anti-CεmX Recombinant Antibody (clone 26H2) (CAT#: FAMAB-0208-CN)
This is a monoclonal CεmX-specific mAb which binds to both mIgE.FcL-expressing CHO cells as well as mIgE.FcL-expressing B cell line Ramos cells. The mAb 26H2 binds to the middle part of CεmX.
Specific Inquiry
FC
Figure 1 The binding of anti-CεmX mAbs to CHO or Ramos lines that express mIgE.FcL or mIgE.FcS.
mIgE.FcS-expressing CHO, mIgE.FcLexpressing CHO, mIgE.FcS-expressing Ramos, or mIgE.FcL-expressing Ramos cells were incubated with 10 mg/ml mouse anti-CεmX mAbs 26H2, followed by staining with FITClabeled rabbit F(ab9)2 specific for mouse IgG.
Chen, J. B., Wu, P. C., Hung, A. F. H., Chu, C. Y., Tsai, T. F., Yu, H. M., ... & Chang, T. W. (2010). Unique epitopes on CεmX in IgE–B cell receptors are potentially applicable for targeting IgE-committed B cells. The journal of immunology, 184(4), 1748-1756.
WB
Figure 2 Induction of apoptosis by chimeric anti-CεmX mAbs.
The cleavage of caspase 3 and PARP in mIgE.FcL-expressing Ramos cells upon treatment with 26H2. Western blot analysis of 30 mg of whole cell lysates with Abs specific for caspase 3 and PARP.
Chen, J. B., Wu, P. C., Hung, A. F. H., Chu, C. Y., Tsai, T. F., Yu, H. M., ... & Chang, T. W. (2010). Unique epitopes on CεmX in IgE–B cell receptors are potentially applicable for targeting IgE-committed B cells. The journal of immunology, 184(4), 1748-1756.
FC
Figure 3 Induction of apoptosis by chimeric anti-CεmX mAbs.
Induction of apoptosis by c4B12 or c26H2 in the absence or presence of secondary Abs. mIgE.FcL-expressing Ramos cells (5×105 cells/ml) were incubated with 1 mg/mlc 26H2 for 1 h at 37°C, followed by incubation with or without 10 mg/ml goat F(ab9)2 specific for Fc of human IgG (2nd Abs), or 2nd Abs along for 24 h.
Chen, J. B., Wu, P. C., Hung, A. F. H., Chu, C. Y., Tsai, T. F., Yu, H. M., ... & Chang, T. W. (2010). Unique epitopes on CεmX in IgE–B cell receptors are potentially applicable for targeting IgE-committed B cells. The journal of immunology, 184(4), 1748-1756.
Apop
Figure 4 The effects of z-VAD-fmk on anti-CεmX-induced apoptosis.
The mAbs, cBAT123 and cB1-8, were control Abs. Data are means 6 SD from three experiments.
Chen, J. B., Wu, P. C., Hung, A. F. H., Chu, C. Y., Tsai, T. F., Yu, H. M., ... & Chang, T. W. (2010). Unique epitopes on CεmX in IgE–B cell receptors are potentially applicable for targeting IgE-committed B cells. The journal of immunology, 184(4), 1748-1756.
Specifications
- Immunogen
- MIgE.Fc recombinant protein (mIgE.FcL)
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric (mouse/human) IgG1, κ
- Specificity
- Human CεmX
- Species Reactivity
- Human
- Clone
- 26H2
- Applications
- ELISA, WB, FC, Apop, Cyt
- Related Disease
- Various allergic diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Apoptosis and Cytotoxicity.
Target
- Alternative Names
- CεmX
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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See other products for "Clone 26H2"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-058 | Human Anti-CεmX Recombinant Antibody (PABL-058) | ELISA, FuncS | Human IgG |
| FAMAB-0209-CN | Human Anti-CεmX Recombinant Antibody (clone a20) | ELISA, WB, FC, Apop, Cyt | Chimeric (mouse/human) IgG1, κ |
| FAMAB-0210-CN | Human Anti-CεmX Recombinant Antibody (clone 1A8) | ELISA, WB, FC, Apop, Cyt | Chimeric (mouse/human) IgG1, κ |
| FAMAB-0211-CN | Human Anti-CεmX Recombinant Antibody (clone 3A6) | ELISA, WB, FC, Apop, Cyt | Chimeric (mouse/human) IgG1, κ |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-058 | Human Anti-CεmX Recombinant Antibody; scFv Fragment (PSBL-058) | ELISA, FuncS | Human scFv |
| FAMAB-0208-CN-S(P) | Mouse Anti-CεmX Recombinant Antibody (clone 26H2); scFv Fragment | ELISA, WB, FC, Apop, Cyt | Mouse scFv |
| FAMAB-0209-CN-S(P) | Mouse Anti-CεmX Recombinant Antibody (clone a20); scFv Fragment | ELISA, WB, FC, Apop, Cyt | Mouse scFv |
| FAMAB-0210-CN-S(P) | Mouse Anti-CεmX Recombinant Antibody (clone 1A8); scFv Fragment | ELISA, WB, FC, Apop, Cyt | Mouse scFv |
| FAMAB-0211-CN-S(P) | Mouse Anti-CεmX Recombinant Antibody (clone 3A6); scFv Fragment | ELISA, WB, FC, Apop, Cyt | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-058 | Human Anti-CεmX Recombinant Antibody; Fab Fragment (PFBL-058) | ELISA, FuncS | Human Fab |
| FAMAB-0208-CN-F(E) | Human Anti-CεmX Recombinant Antibody (clone 26H2); Fab Fragment | ELISA, WB, FC, Apop, Cyt | Chimeric (mouse/human) Fab |
| FAMAB-0209-CN-F(E) | Human Anti-CεmX Recombinant Antibody (clone a20); Fab Fragment | ELISA, WB, FC, Apop, Cyt | Chimeric (mouse/human) Fab |
| FAMAB-0210-CN-F(E) | Human Anti-CεmX Recombinant Antibody (clone 1A8); Fab Fragment | ELISA, WB, FC, Apop, Cyt | Chimeric (mouse/human) Fab |
| FAMAB-0211-CN-F(E) | Human Anti-CεmX Recombinant Antibody (clone 3A6); Fab Fragment | ELISA, WB, FC, Apop, Cyt | Chimeric (mouse/human) Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-114 | Recombinant Human Anti-IgE Antibody (47H4) | Neut, FuncS | IgG |
| PABX-114-F (E) | Recombinant Human Anti-IgE Antibody Fab Fragment (47H4) | Neut, FuncS | Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-114-S (P) | Recombinant Human Anti-IgE Antibody scFv Fragment (47H4) | Neut, FuncS | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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