Mouse Anti-C. parvum P23 Recombinant Antibody (clone 1E10) (CAT#: FAMAB-0100JF)
Recombinant mouse monoclonal antibody specially binds to Cryptosporidium parvum P23. This anti-P23 antibody 1E10 can be useful in defending against Cryptosporidium.
Specific Inquiry
WB
Figure 1 Western immunoblot demonstrating the monomeric-polymeric states of anti-C. parvum IgA MAbs.
The values on the left are molecular masses in kilodaltons.
Enriquez, F. J., & Riggs, M. W. (1998). Role of immunoglobulin A monoclonal antibodies against P23 in controlling murine Cryptosporidium parvum infection. Infection and Immunity, 66(9), 4469-4473.
IF
Figure 2 Oocysts were analyzed with serum from rabbit immunized with recombinant P23 protein.
Negative control with rabbit serum before immunization with recombinant P23.
Omidian, Z., Ebrahimzadeh, E., Shahbazi, P., Asghari, Z., & Shayan, P. (2014). Application of recombinant Cryptosporidium parvum P23 for isolation and prevention. Parasitology research, 113(1), 229-237.
Dot
Figure 3 P23 was dotted on the lower site of the nitrocellulose membranes.
As a negative control, Theileria sp. antigen was dotted on the upper site of the corresponding membranes.
Omidian, Z., Ebrahimzadeh, E., Shahbazi, P., Asghari, Z., & Shayan, P. (2014). Application of recombinant Cryptosporidium parvum P23 for isolation and prevention. Parasitology research, 113(1), 229-237.
FuncS
Figure 4 The mice in the test group were treated with anti-P23 IgY and those in the control group were treated with Igy isolated from non-immune eggs.
Oocyst shedding was then monitored from day 3 for 7 days.
Omidian, Z., Ebrahimzadeh, E., Shahbazi, P., Asghari, Z., & Shayan, P. (2014). Application of recombinant Cryptosporidium parvum P23 for isolation and prevention. Parasitology research, 113(1), 229-237.
Specifications
- Immunogen
- P23 emulsified in monophosphoryl A-trehalose dimycolate adjuvan
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Cryptosporidium parvum P23
- Species Reactivity
- C. parvum
- Clone
- 1E10
- Applications
- WB, IF, FuncS, Dot
- Related Disease
- Cryptosporidium parvum infection
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The C. parvum P23 antibody has been reported in applications of Western Blot, Immunofluorescence, Dot blot, Functional Assay.
Target
- Alternative Names
- 23kDa sporozoite surface pellicle protein; P23
- Function
- Neutralization
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0100JF-F(E) | Mouse Anti-C. parvum P23 Recombinant Antibody (clone 1E10); Fab Fragment | IB, ELISA, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0100JF-S(P) | Mouse Anti-C. parvum P23 Recombinant Antibody (clone 1E10); scFv Fragment | IB, ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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