Rabbit Anti-C4A Recombinant Antibody (clone R05-1T2) (CAT#: VS3-XY3284)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human C4A

• Species Reactivity
• Human

• Clone
• R05-1T2

• Applications
• WB, IHC-P, FC

• Related Disease
• Complement component 4A deficiency (C4AD); Systemic lupus erythematosus (SLE)

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IHC: 1/50-1/100
  FC: 1/50-1/100

• Alternative Names
• C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2

• Gene ID
• 720

• UniProt ID
• P0C0L4

• Long Name
• Complement C4A

• Cellular Localization
• Cell projection, Secreted, Synapse

• Post Translation Modifications
• Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

• Protein Refseq
• NP_009224.2; NP_001239133.1

• Function
• Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody

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