Human Anti-CA19-9 Recombinant Antibody (HPAB-0038-WJ)
CAT#: HPAB-0038-WJ
This product is a human monoclonal IgG1 antibody targeting sialyl-Lewis(a) (sLea), an epitope present on the CA19-9 cancer antigen. On the basis of the potential of sLea as a target for immune attack and their affinity, specificity, and effector functions, this antibody has clinical utility in the treatment of cancer.
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human sLea of CA19-9
- Species Reactivity
- Human
- Applications
- ELISA, IHC, IRMA, IF, Cyt, FC
- Related Disease
- Colon cancer, Pancreatic adenocarcinoma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CA19-9 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunoradiometric Assay, Immunofluorescence, Cytotoxicity, Flow Cytometry.
For FC: sLeᵃ positive or negative tumor cell lines (0.5×10⁶ cells per condition) were washed in PBS/2% FBS (PBSF). Test or control human mAb was then added (1-2μg/ml in complete medium) and incubated on ice for 30 minutes. After washing in PBSF, the cells were incubated with Alexa-488 anti-human IgG (Fcγ) or anti-human IgM (μ) for 30 minutes on ice. Cells were washed twice in PBSF and analyzed by flow cytometry using the Guava Personal Cell Analysis-96 (PCA-96) System. Colo205-luc cells were incubated with 2 μg/ml primary antibody followed by staining with secondary antibodies, and analyzed on a Becton Dickinson FC Advantage IV instrument using FlowJo 7.2.4 software.
For ELISA: For the sLeᵃ ELISA, plates were coated either with 1 μg/ml of sLeᵃ-HSA conjugate, monovalent biotinylated-sLeᵃ, or with polyvalent biotinylated sLeᵃ-PAA captured on Neutravidin coated plates. Uncoated wells (PBS) and human serum albumin (HSA) coated wells were used as controls. Bound antibodies were initially detected with HRP-labeled goat anti-human IgA+G+M and positive wells were subsequently probed with IgG-Fc or IgM specific secondary antibodies to determine isotypes.
Target
- Alternative Names
- CA19-9; Human CA19-9
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0038-WJ, RRID: AB_3111348)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CA19-9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0267MZ | Mouse Anti-CA19-9 Recombinant Antibody (clone 1117-OS-20-10) | IHC | Mouse IgG1 |
| MOB-318YJL | Mouse Anti-CA19-9 Recombinant Antibody (clone 116-NS-19-9) | IHC-P, ICC, IF, FC | Mouse IgG1, κ |
| VS3-CJ109 | Mouse Anti-CA19-9 Recombinant Antibody (VS3-CJ109) | IHC, IF | Mouse IgG |
| VS3-FY2602 | Mouse Anti-CA19-9 Recombinant Antibody (VS3-FY2602) | IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-WJ-S(P) | Human Anti-CA19-9 Recombinant Antibody scFv Fragment (HPAB-0038-WJ-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-WJ-F(E) | Human Anti-CA19-9 Recombinant Antibody Fab Fragment (HPAB-0038-WJ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS1-YJ20 | Mouse Anti-CA19-9 Recombinant Antibody (clone 121SLE) | FC, ELISA, IHC-P, IHC-fr, WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY53 | Human Anti-CA19-9 (clone 5B1) scFv-Fc Chimera | ELISA, IHC, IRMA, IF, Cyt, FC, BI | Human IgG1, scFv-Fc |
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