Human Anti-CA19-9 Recombinant Antibody (HPAB-0038-WJ) (CAT#: HPAB-0038-WJ)

• Host Species
• Human

• Type
• Human IgG1

• Specificity
• Human sLea of CA19-9

• Species Reactivity
• Human

• Applications
• ELISA, IHC, IRMA, IF, Cyt, FC

• Related Disease
• Colon cancer, Pancreatic adenocarcinoma

• Purity
• >95% as determined by SDS-PAGE and HPLC analysis

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• No preservatives

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• The CA19-9 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunoradiometric Assay, Immunofluorescence, Cytotoxicity, Flow Cytometry.
  For FC: sLeᵃ positive or negative tumor cell lines (0.5×10⁶ cells per condition) were washed in PBS/2% FBS (PBSF). Test or control human mAb was then added (1-2μg/ml in complete medium) and incubated on ice for 30 minutes. After washing in PBSF, the cells were incubated with Alexa-488 anti-human IgG (Fcγ) or anti-human IgM (μ) for 30 minutes on ice. Cells were washed twice in PBSF and analyzed by flow cytometry using the Guava Personal Cell Analysis-96 (PCA-96) System. Colo205-luc cells were incubated with 2 μg/ml primary antibody followed by staining with secondary antibodies, and analyzed on a Becton Dickinson FC Advantage IV instrument using FlowJo 7.2.4 software.
  For ELISA: For the sLeᵃ ELISA, plates were coated either with 1 μg/ml of sLeᵃ-HSA conjugate, monovalent biotinylated-sLeᵃ, or with polyvalent biotinylated sLeᵃ-PAA captured on Neutravidin coated plates. Uncoated wells (PBS) and human serum albumin (HSA) coated wells were used as controls. Bound antibodies were initially detected with HRP-labeled goat anti-human IgA+G+M and positive wells were subsequently probed with IgG-Fc or IgM specific secondary antibodies to determine isotypes.

• Alternative Names
• CA19-9; Human CA19-9

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