Anti-CA9 (clone M75) Recombinant Antibody Coupled Liposome (VS-1024-FY34) (CAT#: VS-1024-FY34)

• Potential Clinical Applications
• Lung cancer

• Immunoliposomes Method
• Immunoliposomes were created using a modified post-insertion technique. DSPE-PEG-MAL micelles were formed by hydrating a lipid film in PBS, followed by thiolation of the anti-CA IX antibody using DTT. Unwanted reducing agents were removed through ethyl acetate extraction. Antibody-conjugated micelles were incubated with TPL-Lips to create CA IX-TPL-Lips, while unmodified micelles were used to form non-targeted TPL-Lips. Sepharose CL-4B gel filtration was utilized to eliminate unincorporated liposomes.

• Clone
• M75

• Antibody Type
• IgG1

• Antibody Host
• Mouse

• Antibody Reactivity
• Human

• Antibody Description
• This mouse monoclonal antibody specifically targets the human CA9 protein and can be utilized in various applications, including ELISA (dilution of 1:10,000), Western Blot (dilution range of 1:500 to 1:2000), and Flow Cytometry (dilution range of 1:200 to 1:400). The immunogen used to generate this antibody is a recombinant protein corresponding to amino acids 37-186 of the human CA9, which was expressed in E. coli.

• Storage
• See in the COA

• Storage Shelf Time
• See in the COA

• Target
• CA9

• Introduction
• Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.

• Alternative Names
• MN; CAIX

• Full Name
• carbonic anhydrase 9

• Gene ID
• 768

• UniProt ID
• Q16790

• Sequence Similarities
• Belongs to the alpha-carbonic anhydrase family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Asn-346 bears high-mannose type glycan structures. ( Q16790-CAH9_HUMAN )
  Glycosylation at Thr115 and Asn346 ( NX_Q16790 [NX_Q16790-1] )
  Modification sites at PhosphoSitePlus ( Q16790 )
  Glycosylation from GlyGen (Q16790) 3 sites, 4 O-linked glycans (2 sites)

• Protein Refseq
• XP_047279805.1; XP_047279806.1; XP_054219735.1; XP_054219736.1; NP_001207.2

• Function
• Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e.
  bicarbonate and hydrogen ions).

• PABZ-017
• Mouse Anti-CA9 Recombinant Antibody (clone M75)

• PFBZ-017
• Mouse Anti-CA9 Recombinant Antibody (clone M75); Fab Fragment

• PSBZ-017
• Mouse Anti-CA9 Recombinant Antibody (clone M75); scFv Fragment

• PABZ-017-mRNA
• Mouse Anti-CA9 Antibody (clone M75), mRNA

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