Rabbit Anti-CALB2 Recombinant Antibody (VS-0223-WK14)
CAT#: VS-0223-WK14
The anti-CALB2 antibody was generated by immunizing Rabbit. The immunogen is recombinant protein corresponding to amino acids 1 to 271 from mouse Calretinin. This antibody has been tested for use in Western Blot (1/1000), Immunocytochemistry (1/500) and Immunohistochemistry (1/500).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7305/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7306/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7305/1895_C10_2_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7305/18325_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7305/18325_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7305/18325_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7305/18325_A_6_6.jpg
Normal Tissue
Figure 8 Soft tissue
Peripheral nerve
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7305/18325_B_1_8.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7305/18325_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (CAPAN-2, HEL, HMC-1, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172137-CALB2
❮
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Specifications
- Immunogen
- Recombinant protein corresponding to AA 1 to 271 from mouse Calretinin
- Host Species
- Rabbit
- Derivation
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse CALB2
- Species Reactivity
- Rat, Mouse, Human, Zebrafish
- Applications
- WB, ICC, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Lyophilized powder
- Storage
- Aliquot and store at -20°C to -80°C for 1 year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000), Immunocytochemistry (1:500) and Immunohistochemistry (1:500).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "CALB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0288CT | Mouse Anti-CALB2 Recombinant Antibody (clone 6B6) | IHC-P | Mouse IgG1 |
| MRO-0222-CN | Rabbit Anti-CALB2 Recombinant Antibody (clone JM12-93) | WB, IHC | Rabbit IgG |
| ZG-0063J | Mouse Anti-CALB2 Recombinant Antibody (ZG-0063J) | IHC-P, IF | Mouse IgG |
| ZG-0064J | Mouse Anti-CALB2 Recombinant Antibody (ZG-0064J) | IHC-P, IF | Mouse IgG |
| VS3-FY179 | Recombinant Mouse Anti-CALB2 Antibody (clone 2B5-1D3-9H3) | IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0437 | Hi-Affi™ Rabbit Anti-CALB2 Recombinant Antibody (clone DS437AB) | IHC | Rabbit IgG |
| MOR-0104-FY | Rabbit Anti-CALB2 Recombinant Antibody (clone AFY0075) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY294 | Anti-CALB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY933 | Anti-Mouse CALB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY932 | Anti-Human CALB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY934 | Anti-Rat CALB2 Immunohistochemistry Kit | IHC |
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