CytoStream™ Mouse Anti-CAPN1 Recombinant Antibody (VS13-YC119) (CAT#: VS13-YC119)
The CytoStream™ Mouse Anti-CAPN1 Recombinant Antibody is specific for human, mouse and rat CAPN1. CAPN1 encodes the large subunit of calpain 1, a non-lysosomal cysteine protease involved in cellular processes, with multiple transcript variants encoding distinct enzyme isoforms. The anti-CAPN1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/if_selected.jpg
Figure 2 High expression in tonsil
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_4_8_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_4_8_val_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_7_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_4_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_4_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_9_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (RT4)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000014216-CAPN1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000014216-CAPN1
Specifications
- Immunogen
- Recombinant human Calpain 1, aa 1-714.
- Host Species
- Mouse
- Type
- Mouse IgM
- Specificity
- Human, Mouse and Rat CAPN1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Spastic paraplegia, autosomal recessive (SPG76)
Product Property
- Clonality
- Monoclonal
- Purification
- Protein L purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 50% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Immunocytochemistry (ICC): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Calpain 1; Cell Proliferation-Inducing Gene 30 Protein; Calcium-Activated Neutral Proteinase 1; Calpain 1, (Mu/I) Large Subunit; Calpain-1 Large Subunit; Micromolar-Calpain; Calpain Mu-Type; EC 3.4.22.52; CANP 1; CANPL1;
- Gene ID
- 823
- UniProt ID
- P07384
- Long Name
- Calpain 1
- Cellular Localization
- Cell membrane; Cytoplasm
- Protein Refseq
- XP_006718761.1
- Function
- Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'.
Cleaves and activates caspase-7.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2490z | Mouse Anti-CAPN1 Recombinant Antibody (clone 36A11) | WB, ELISA, FC, IHC | Mouse IgG1, κ |
| MRO-1712-CN | Rabbit Anti-CAPN1 Polyclonal Antibody (MRO-1712-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0680F | Mouse Anti-CAPN1 Recombinant Antibody (clone 15C10) | WB, IP, ELISA | Mouse IgG1 |
| VS3-CJ468 | Mouse Anti-CAPN1 Recombinant Antibody (VS3-CJ468) | WB, IHC-P, FC | Mouse IgG1, κ |
| VS7-HM290 | Mouse Anti-CAPN1 Recombinant Antibody (clone 1E6G9) | FCM | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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