Mouse Anti-CARD9 Recombinant Antibody (VS3-FY2646)
CAT#: VS3-FY2646
The anti-CARD9 antibody is a mouse recombinant monoclonal antibody, which has been intricately crafted to target the CARD9 (N-term). The calculated molecular weight of the antigen is 62.2 kDa. It shows capability of cross-reacting with human, mouse and rat. This monoclonal antibody is potential for conducting research on Immunodeficiency 103 Fungal Infections and Phaeohyphomycosis. This monoclonal antibody can be used in Western Blot.
Gene Expression
Normal Tissue
Figure 1 High expression in spleen
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59502/133359_B_8_4_val_selected.jpg
Normal Tissue
Figure 2 Low expression in cerebral cortex
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59502/133359_B_9_5_val_selected.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59502/133359_A_6_6.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59502/133359_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59502/133359_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEL, HMC-1, NB-4, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187796-CARD9
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Caspase recruitment domain family member 9 of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 62.2 kDa.
- Related Disease
- Immunodeficiency 103 Fungal Infections and Phaeohyphomycosis
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Target
- Alternative Names
- Caspase Recruitment Domain Family Member 9; HCARD9; Caspase Recruitment Domain-Containing Protein 9; Caspase Recruitment Domain Family, Member 9; CANDF2
- Gene ID
- 64170
- UniProt ID
- Q9H257
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated at Thr-231 by PRKCD downstream of C-type lectin receptors activation: phosphorylation promotes interaction with BCL10, followed by activation of NF-kappa-B and MAP kinase p38 pathways (By similarity).
Phosphorylated at Thr-531 and Thr-533 by CK2 following interaction with VHL, leading to inhibit the ability to activate NF-kappa-B (By similarity).
Ubiquitinated at Lys-125 via 'Lys-27'-linked ubiquitin by TRIM62 downstream of C-type lectin receptors activation; leading to CARD9 activation, followed by activation of NF-kappa-B and MAP kinase p38 pathways.
Deubiquitinated at Lys-125 by USP15, inhibiting CARD9.
Ubiquitination at Lys125
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_434700.2; NP_434701.1
- Function
- Upon activation, CARD9 homooligomerizes to form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10 and subsequent recruitment of MALT1: this leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2646. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
NLR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CARD9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0468 | Hi-Affi™ Rabbit Anti-CARD9 Recombinant Antibody (clone DS468AB) | WB, IHC | Rabbit IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
-2.png)
-2.png)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: FC, FRET, Internalization
Application: WB, ELISA, Neut, FuncS
Application: Neut, ELISA, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, PD, Activ, PK, Stim
Application: ELISA, Block, WB, FC, IP
Application: ELISA, Neut
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
