Human Anti-Cardiolipin Recombinant Antibody (clone B3)
CAT#: FAMAB-0460WJ
This product is a human monoclonal antibody that is specific for Human Cardiolipin. This antibody can be used in a variety of applications, such as ELISA, WB.
Tested Data
Published Data
SDS-PAGE
Figure 1 Human Anti-Cardiolipin Recombinant Antibody (FAMAB-0460WJ) in SDS-PAGE
Lane 1: Reduced
Lane 2: Non-reduced
SEC-HPLC
Figure 2 Human Anti-Cardiolipin Recombinant Antibody (FAMAB-0460WJ) in HPLC
The purity of FAMAB-0460WJ was greater than 95% as determined by SEC-HPLC.
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ELISA
Figure 1 Light chains expressed with IS4 variable heavy chainregion (VH).
Presented as concentration of IgG in the supernatant versus optical density (OD) at 405 nm in the anti-cardiolipin ELISA.
Giles, I., Lambrianides, N., Latchman, D., Chen, P., Chukwuocha, R., Isenberg, D., & Rahman, A. (2004). The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin. Arthritis Res Ther, 7(1), 1-10.
ELISA
Figure 2 Light chains expressed with B3VH.
Presented as concentration of IgG in the supernatant versus optical density (OD) at 405 nm in the anti-cardiolipin ELISA.
Giles, I., Lambrianides, N., Latchman, D., Chen, P., Chukwuocha, R., Isenberg, D., & Rahman, A. (2004). The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin. Arthritis Res Ther, 7(1), 1-10.
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Antibody Isotype
- IgG
- Specificity
- Human Cardiolipin
- Species Reactivity
- Human
- Clone
- B3
- Applications
- ELISA, WB
- Related Disease
- Antiphospholipid antibody syndrome
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Cardiolipin
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Customer Review
There are currently no Customer reviews or questions for FAMAB-0460WJ. Click the button above to contact us or submit your feedback about this product.
Excellent for ELISA
I used the B3 clone for my ELISA experiments, and the results were very reliable. The fact that it's a recombinant human IgG1 antibody provided great consistency between batches. The specificity for cardiolipin was high, leading to low background noise in my assays. The provided datasheet was clear and accurate regarding its specifications.
High-Quality Recombinant Antibody
This is a great product. As a human monoclonal antibody, it minimizes potential cross-reactivity issues I've had with other antibodies. It performed exactly as expected in our ELISA setup, targeting cardiolipin effectively. The consistent performance of this recombinant antibody is a significant advantage for the reproducibility of our research studies.
Q&As
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I'm looking for a human monoclonal antibody against cardiolipin for an ELISA assay. Can you confirm the isotype and host of this B3 clone? I need to ensure it's compatible with my secondary antibody.
A: We can confirm that our Anti-Cardiolipin Recombinant Antibody (CAT: FAMAB-0460WJ), clone B3, is a human monoclonal antibody. Specifically, its isotype is Human IgG1 kappa. This recombinant antibody was developed using a human host, making it suitable for various research applications, particularly ELISA as listed on the product sheet.
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Has this anti-cardiolipin antibody been validated for applications other than ELISA? I am interested in its potential use in other immunoassays.
A: Thank you for your inquiry. This specific recombinant antibody, clone B3, has been tested and validated for use in ELISA applications as per our current product data. We currently have performance data for WB. We believe its high specificity for cardiolipin might make it a candidate for other immunoassays, any use outside of the validated ELISA application would need to be optimized by the end-user.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# FAMAB-0460WJ, RRID: AB_3713741)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Cardiolipin"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0251JF | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.72) | WB, ELISA, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0251JF-F(E) | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.72); Fab Fragment | IB, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0251JF-S(P) | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.72); scFv Fragment | IB, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0252JF | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.84) | WB, ELISA, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0252JF-F(E) | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.84); Fab Fragment | IB, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0252JF-S(P) | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.84); scFv Fragment | IB, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0458WJ | Human Anti-Cardiolipin Recombinant Antibody (clone RSP4) | ELISA, WB | Human IgM, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0459WJ | Human Anti-Cardiolipin Recombinant Antibody (clone IS4) | ELISA, WB | Human IgG |
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