Rabbit Anti-CASP1 Recombinant Antibody (VS3-CJ857) (CAT#: VS3-CJ857)

• Immunogen
• Recombinant protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat CASP1

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, IHC, FC

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunohistochemistry, Flow Cytometry.

• Alternative Names
• ICE; P45; IL1BC

• Gene ID
• 834

• UniProt ID
• P29466

• Sequence Similarities
• Belongs to the peptidase C14A family.

• Cellular Localization
• Cell membrane, Cytoplasm, Membrane

• Post Translation Modifications
• The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8.
  Cleavage in the interdomain linker region is required to induce pyroptosis.

• Protein Refseq
• NP_001214.1; NP_001244047.1; NP_001244048.1

• Function
• Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:26375003, PubMed:32051255).
  Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:15326478, PubMed:32051255).
  Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:1574116, PubMed:7876192, PubMed:15498465).
  Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003).
  In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275).
  Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed:22464733).
  Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
  In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547).
  Isoform Delta: Apoptosis inactive.
  Isoform Epsilon: Apoptosis inactive.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody