Rabbit Anti-CASP14 Recombinant Antibody (VS3-CJ875) (CAT#: VS3-CJ875)
This product is a rabbit antibody that recognizes human, mouse, and rat CASP14.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line hTCEpi
(Immunofluorescent staining of human cell line hTCEpi shows localization to nucleoplasm, cytosol & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27062/1790_F1_6_selected.jpg
(Immunofluorescent staining of human cell line hTCEpi shows localization to nucleoplasm, cytosol & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27062/1790_F1_6_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27062/214_D7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27062/214_D7_1_blue_red_green.jpg
Figure 3 RNA cell line category: Cell line enriched (hTCEpi)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105141-CASP14
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105141-CASP14
Figure 3 RNA cell line category: Cell line enriched (hTCEpi)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105141-CASP14
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105141-CASP14
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat CASP14
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- ARCI12
- Gene ID
- 23581
- UniProt ID
- P31944
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Maturation by proteolytic processing appears to be a two-step process. The precursor is processed by KLK7 to yield the p20/p8 intermediate form which acts on the precursor to yield the p17/p10 mature form (PubMed:22825846).
Initially, cleavage between Ile-152 and Lys-153 has been proposed to yield the large and small subunits of the active enzyme (PubMed:12200134).
- Protein Refseq
- NP_036246.1
- Function
- Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625).
Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity).
In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512).
Involved in processing of prosaposin in the epidermis (By similarity).
May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097).
Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1326z | Mouse Anti-CASP14 Recombinant Antibody (clone 32D12) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-0325CT | Mouse Anti-CASP14 Recombinant Antibody (clone EML1426) | WB | Mouse IgG2a |
| MRO-0239-CN | Rabbit Anti-CASP14 Recombinant Antibody (clone CBACN-088) | WB, IF, IHC, IP, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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