Mouse Anti-CASP3 Recombinant Antibody (clone 1F12) (CAT#: VS3-WK1585)

• Immunogen
• Synthesized peptide derived from human TGF β

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human CASP3

• Species Reactivity
• Human, Mouse, Rat

• Clone
• 1F12

• Applications
• IHC-P, WB

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• PBS, 0.5% BSA, 50% Glycerol

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• IHC-P: 1/50-1/200
  WB: 1/1000-1/2000

• Alternative Names
• Caspase 3; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Apopain; CASP-3

• Gene ID
• 836

• UniProt ID
• P42574

• Long Name
• Caspase 3

• Sequence Similarities
• Belongs to the peptidase C14A family.

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits (PubMed:7596430, PubMed:8755496, PubMed:35338844, PubMed:35446120).
  Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease (PubMed:7596430, PubMed:8755496).
  Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa (PubMed:7596430, PubMed:8755496).
  S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP3 processing, preventing CASP3 activation and ability to recognize and cleave substrates.

• Protein Refseq
• NP_004337.2; NP_116786.1

• Function
• Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis.
  Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins.

Secondary Antibody

• 2RD-A1514-YJ
• Sheep Anti-Mouse IgG-heavy and light chain Secondary antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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