Rabbit Anti-CASP6 Recombinant Antibody (clone R04-8C2)
CAT#: VS3-XY3289
This antibody is a recombinant rabbit antibody targeting the CASP6 (Caspase 6), which acts as a regulator of liver damage by promoting hepatocyte apoptosis. It reacts with human, mouse and rat CASP6. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/20, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11337/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3775/if_selected.jpg
Normal Tissue
Figure 3 Small intestine
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11337/29069_A_5_2_val_selected.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_7_5.jpg
Normal Tissue
Figure 5 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_4_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_7_8.jpg
Normal Tissue
Figure 7 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_9_3.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138794-CASP6
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CASP6
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-8C2
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Huntington Disease; Colorectal Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- MCH2; CSP-6; caspase-6
- Gene ID
- 839
- UniProt ID
- P55212
- Long Name
- Caspase 6
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated by NUAK1; phosphorylation inhibits self-activation.
Phosphorylation at Ser-257 by AMP-activated protein kinase (PRKAA1 or PRKAA2) inhibits autocleavage, preventing caspase activation, thereby preventing hepatocyte apoptosis.
Palmitoylation by ZDHHC17 blocks dimerization and subsequent activation, leading to inhibit the cysteine protease activity.
- Protein Refseq
- NP_001217.2; NP_116787.1
- Function
- Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity. Acts as a non-canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation. Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MOR-0477 | Hi-Affi™ Rabbit Anti-CASP6 Recombinant Antibody (clone DS477AB) | FC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS7-HM296 | Mouse Anti-CASP6 Recombinant Antibody (clone 3B7C3) | FCM | Mouse IgG2a |
| CAT | Product Name | Application | Type |
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| VS-0424-XY42 | AbPlus™ Anti-CASP6 Magnetic Beads (CBACN-091) | IP, Protein Purification |
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