Rabbit Anti-CASP7 Recombinant Antibody (VS3-CJ1043)
CAT#: VS3-CJ1043
This product is a rabbit antibody that recognizes human CASP7.
Gene Expression
Normal Tissue
Figure 1 Small intestine
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25563/59600_A_4_2.jpg
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CASP7
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
- Gene ID
- 840
- UniProt ID
- P55210
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Post Translation Modifications
- Cleavage by different proteases, such as granzyme B (GZMB), caspase-1 (CASP1), caspase-8 (CASP8), caspase-9 (CASP9) or caspase-10 (CASP10) generate the two active subunits (PubMed:9852092, PubMed:12824163, PubMed:16352606, PubMed:16916640, PubMed:35338844, PubMed:35446120).
Its involvement in different programmed cell death processes is probably specified by the protease that activates CASP7 (PubMed:9852092, PubMed:16916640).
Cleaved and activated by initiator caspases (CASP8, CASP9 and/or CASP10), leading to execution phase of apoptosis (PubMed:16352606, PubMed:16916640, PubMed:21555521, PubMed:27889207).
Cleavage and maturation by GZMB regulates granzyme-mediated programmed cell death (By similarity).
Cleaved and activated by CASP1 in response to bacterial infection (By similarity).
Propeptide domains can also be cleaved efficiently by CASP3 (PubMed:8755496).
Active heterodimers between the small subunit of caspase-7 and the large subunit of CASP3, and vice versa, also occur (PubMed:8755496).
Also cleaved at the N-terminus at alternative sites by CAPN1, leading to its activation (PubMed:19617626).
Phosphorylation at Ser-30 and Ser-239 by PAK2 inhibits its activity (PubMed:21555521, PubMed:27889207).
Phosphorylation at Ser-30 prevents cleavage and activation by initiator caspase CASP9, while phosphorylation at Ser-239 prevents thiol protease activity by preventing substrate-binding (PubMed:21555521, PubMed:27889207).
(Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP7 processing, preventing CASP7 activation and ability to recognize and cleave substrates.
- Protein Refseq
- NP_001218.1; NP_001253985.1; NP_001253986.1
- Function
- Thiol protease involved in different programmed cell death processes, such as apoptosis, pyroptosis or granzyme-mediated programmed cell death, by proteolytically cleaving target proteins.
Has a marked preference for Asp-Glu-Val-Asp (DEVD) consensus sequences, with some plasticity for alternate non-canonical sequences.
Its involvement in the different programmed cell death processes is probably determined by upstream proteases that activate CASP7 (By similarity).
Acts as an effector caspase involved in the execution phase of apoptosis: following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of proteins, such as CLSPN, PARP1, PTGES3 and YY1.
Compared to CASP3, acts as a minor executioner caspase and cleaves a limited set of target proteins (PubMed:18723680).
Acts as a key regulator of the inflammatory response in response to bacterial infection by catalyzing cleavage and activation of the sphingomyelin phosphodiesterase SMPD1 in the extracellular milieu, thereby promoting membrane repair (PubMed:21157428).
Regulates pyroptosis in intestinal epithelial cells: cleaved and activated by CASP1 in response to S.typhimurium infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of gasdermin-D (GSDMD) pores (By similarity).
Regulates granzyme-mediated programmed cell death in hepatocytes: cleaved and activated by granzyme B (GZMB) in response to bacterial infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of perforin (PRF1) pores (By similarity).
Following cleavage by CASP1 in response to inflammasome activation, catalyzes processing and inactivation of PARP1, alleviating the transcription repressor activity of PARP1 (PubMed:22464733).
Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (By similarity).
Cleaves and activates sterol regulatory element binding proteins (SREBPs) (PubMed:8643593).
Cleaves phospholipid scramblase proteins XKR4, XKR8 and XKR9 (By similarity).
In case of infection, catalyzes cleavage of Kaposi sarcoma-associated herpesvirus protein ORF57, thereby preventing expression of viral lytic genes (PubMed:20159985).
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Related Signaling Pathways
TNF Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CASP7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1859z | Mouse Anti-CASP7 Recombinant Antibody (clone 10F11) | WB, IHC | Mouse IgG |
| ZG-0867F | Mouse Anti-CASP7 Recombinant Antibody (clone MCH3 14 1-11) | WB | Mouse IgG |
| ZG-0868F | Mouse Anti-CASP7 Recombinant Antibody (clone MCH3-5) | WB | Mouse IgG |
| VS3-XY165 | Mouse Anti-CASP7 Recombinant Antibody (clone 10H12B10) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY1983 | Rabbit Anti-CASP7 Recombinant Antibody (clone R06-0F6) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B072(A) | Recombinant Anti-human CASP7 Intrabody [(D-Arg)9] | WB, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B072(G) | Recombinant Anti-human CASP7 Intrabody [+36 GFP] | WB, ICC, Neut, FuncS | scFv-(+36GFP) |
| IAB-B072(T) | Recombinant Anti-human CASP7 Intrabody [Tat] | IF, FC, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0478 | Hi-Affi™ Rabbit Anti-CASP7 Recombinant Antibody (clone DS478AB) | IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1356 | AbPlus™ Anti-CASP7 Magnetic Beads (VS-0724-YC1356) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY320 | Anti-CASP7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1007 | Anti-Mouse CASP7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1006 | Anti-Human CASP7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1008 | Anti-Rat CASP7 Immunohistochemistry Kit | IHC |
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