Mouse Anti-CASP8 Recombinant Antibody (clone 2G12) (CAT#: ZG-0072J)
This product is a mouse antibody that recognizes CASP8.
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Custom Production
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IF
Figure 1 Mouse Anti-CASP8 Recombinant Antibody (clone 2G12) in IF
Immunofluorescence analysis of Hela cell. 1.ERα Polyclonal Antibody(red) was diluted at 1:200 (4°C overnight). Caspase-8 Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
IHC
Figure 2 Mouse Anti-CASP8 Recombinant Antibody (clone 2G12) in IHC
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Caspase-8 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 3 Mouse Anti-CASP8 Recombinant Antibody (clone 2G12) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1.Caspase-8 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
WB
Figure 4 Mouse Anti-CASP8 Recombinant Antibody (clone 2G12) in WB
Immunofluorescence analysis of Mouse-liver tissue. 1.Caspase-8 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2 Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
IF
Figure 5 Mouse Anti-CASP8 Recombinant Antibody (clone 2G12) in IF
Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue using Caspase-8 Monoclonal Antibody.
Specifications
- Immunogen
- Recombinant Protein of Caspase-8
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CASP8
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 2G12
- Applications
- WB, IF/ICC, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Paraffin.
Target
- Alternative Names
- CASP8; caspase 8, apoptosis-related cysteine peptidase; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; caspase-8; FADD-like ICE; MACH-alpha-1/2/3 protein; apoptotic protease Mch-5; MACH-beta-1/2/3/4 protein; apoptotic cysteine protease; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; FADD-homologous ICE/CED-3-like protease; caspase 8, apoptosis-related cysteine protease
- Gene ID
- 841
- UniProt ID
- Q14790
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| BRD-0082MZ | Chicken Anti-Caspase 8 Polyclonal IgY | WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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