Mouse Anti-CBFA2T3 Recombinant Antibody (VS3-CJ171) (CAT#: VS3-CJ171)

• Immunogen
• Recombinant protein between 418-653 amino acids from human CBFA2T3

• Host Species
• Mouse

• Specificity
• Human CBFA2T3

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000-1:2000).

• Alternative Names
• ETO2; MTG16; MTGR2; ZMYND4; RUNX1T3

• Gene ID
• 863

• UniProt ID
• O75081

• Sequence Similarities
• Belongs to the CBFA2T family.

• Cellular Localization
• Golgi apparatus, Membrane, Nucleus

• Protein Refseq
• NP_005178.4; NP_787127.1

• Function
• Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199).
  Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453).
  Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway (PubMed:25974097).
  Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (PubMed:23840896, PubMed:25974097).
  Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (By similarity).
  Plays a role in granulocyte differentiation (PubMed:15231665).
  Isoform 2 functions as an A-kinase-anchoring protein (PubMed:11823486).

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS