Rabbit Anti-CBL (pS669) Recombinant Antibody (clone R48-7F-7)
CAT#: VS3-WK2073
This product is a rabbit recombinant antibody that is specific to phosphorylated CBL. The target of this antibody is phospho-CBL (Ser669). The clone R48-7F-7 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of CBL (pSer669) observed in WB experiments using this antibody is 120 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/257_E11_2_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_9_8.jpg
Normal Tissue
Figure 3 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_4_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Round or early spermatids Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110395-CBL
❮
❯
| Sub Cat | Clone | Species Reactivity | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS3-WK2300 | R48-7F-7 | Human, Mouse, Rat | ||||||
| VS3-WK2237 | Human |
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser669 of human, mouse and rat CBL
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- Cbl Proto-Oncogene; Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence; Cbl Proto-Oncogene, E3 Ubiquitin Protein Ligase; Casitas B-Lineage Lymphoma Proto-Oncogene; RING-Type E3 Ubiquitin Transferase CBL; Signal Transduction Protein CBL; RING Finger Protein 55; Proto-Oncogene C-Cbl; Oncogene CBL2; RNF55
- Gene ID
- 867
- UniProt ID
- P22681
- Long Name
- Cbl Proto-Oncogene
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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