Mouse Anti-CBL Recombinant Antibody (VS3-CJ789) (CAT#: VS3-CJ789)

• Immunogen
• KLH-conjugated synthetic peptide between 450-644 amino acids from human CBL

• Host Species
• Mouse

• Type
• Mouse IgG2b, κ

• Specificity
• Human CBL

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS

• Preservative
• 0.09% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:2000).

• Alternative Names
• CBL2; NSLL; C-CBL; RNF55; FRA11B

• Gene ID
• 867

• UniProt ID
• P22681

• Cellular Localization
• Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane

• Post Translation Modifications
• Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity).
  Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK.
  Ubiquitinated, leading to its degradation via the proteasome (PubMed:11896602, PubMed:20094046).
  Ubiquitination is negatively regulated by IFT20 (PubMed:29237719).

• Protein Refseq
• NP_005179.2

• Function
• Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome (PubMed:17094949).
  Ubiquitinates SPRY2 (PubMed:17094949, PubMed:17974561).
  Ubiquitinates EGFR (PubMed:17974561).
  Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).

Isotype Control

• MOB-212YJL
• Mouse IgG2b kappa Isotype Control Antibody (Mopc21)

• MOB-203CQ
• Mouse IgG2b, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS