Human Anti-CBX1 Recombinant Antibody (clone 2506W1345)
CAT#: VS-0625-FY1450
This recombinant antibody is a human IgG1 antibody, generated through recombinant antibody (rAb) phage display. Its specificity and affinity for the CBX1 protein have been thoroughly tested under non-denaturing conditions. While no specific applications are listed, this antibody provides a reliable tool for recognizing CBX1. The antibody targets an epitope located within amino acids 104-174 of the CBX1 protein, making it valuable for studies requiring recognition of native CBX1 or for developing custom assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/610_F6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/617_F6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/613_F6_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108468-CBX1
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- The antibody reacts with human CBX1.
- Species Reactivity
- Human
- Clone
- 2506W1345
- Applications
- ELISA, Immunofluorescence, Immunoprecipitation Mass Spectrometry
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (over 6 months). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- Chromobox 1; Heterochromatin Protein 1 Homolog Beta; Modifier 1 Protein; HP1Hsbeta; P25beta; CBX; M31; Chromobox Homolog 1 (HP1 Beta Homolog Drosophila ); Chromobox Homolog 1 (Drosophila HP1 Beta); Heterochromatin Protein P25 Beta; HP1 Beta Homolog (Drosophila )
- Gene ID
- 10951
- UniProt ID
- P83916
- Long Name
- Chromobox 1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CBX1"
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| MOB-0350CT | Mouse Anti-CBX1 Recombinant Antibody (clone 6B4) | ELISA, FC, IF, WB | Mouse IgG1 |
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| VS11-QX15 | Human Anti-CBX1 Recombinant Antibody (clone RAB-C145) | ELISA, IF, IP-MS, ChIP | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0494 | Hi-Affi™ Rabbit Anti-CBX1 Recombinant Antibody (clone DS494AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1385 | AbPlus™ Anti-CBX1 Magnetic Beads (VS-0724-YC1385) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY324 | Anti-CBX1 Immunohistochemistry Kit | IHC |
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