Mouse Anti-CBX1 Recombinant Antibody (ZG-0442J)
CAT#: ZG-0442J
This product is a mouse antibody that recognizes CBX1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CBX1 Recombinant Antibody (ZG-0442J) in WB
Western Blot analysis using HP1β Monoclonal Antibody against MCF7, HepG2 cell lysate.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/610_F6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/617_F6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/613_F6_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108468-CBX1
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Specifications
- Immunogen
- Purified recombinant human HP1β protein fragments expressed in E.coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CBX1
- Species Reactivity
- Human, Mouse, Rat, Bovine, Chicken, Dog, Pig
- Applications
- WB
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot.
Target
- Alternative Names
- CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta; chromobox protein homolog 1; HP1 beta homolog; chromobox homolog 1 (HP1 beta homolog Drosophila); heterochromatin protein 1 homolog beta; heterochromatin protein 1-beta; heterochromatin protein p25 beta; modifier 1 protein
- Gene ID
- 10951
- UniProt ID
- P83916
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CBX1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-268 | Human Anti-CBX1 Recombinant Antibody (clone D256); Fab Fragment | IF, ELISA, IP-MS, ChIP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0494 | Hi-Affi™ Rabbit Anti-CBX1 Recombinant Antibody (clone DS494AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS3-CJ73 | Mouse Anti-CBX1 Recombinant Antibody (VS3-CJ73) | WB, ELISA | Mouse IgG |
| VS3-XY170 | Mouse Anti-CBX1 Recombinant Antibody (clone 4D10A6) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS7-HM300 | Mouse Anti-CBX1 Recombinant Antibody (clone 2B7D6) | IHC | Mouse IgG1 |
| VS7-HM301 | Mouse Anti-CBX1 Recombinant Antibody (clone 5A3) | WB, ICC, FCM | Mouse IgG1 |
| VS-0625-FY1450 | Human Anti-CBX1 Recombinant Antibody (clone 2506W1345) | ELISA, IF, IP MS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1385 | AbPlus™ Anti-CBX1 Magnetic Beads (VS-0724-YC1385) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY324 | Anti-CBX1 Immunohistochemistry Kit | IHC |
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