Mouse Anti-CBX3 Recombinant Antibody (VS4-WK67)
CAT#: VS4-WK67
This product is a mouse monoclonal antibody that recognizes CBX3 and can be used in ELISA, WB, IHC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/85_C1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows distinct nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1973/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/108_C1_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/87_C1_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/16166_B_9_5.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/16166_B_3_5.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/16166_A_8_3.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/16166_A_8_5.jpg
Normal Tissue
Figure 10 Testis
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Strong
Quantity: <25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/16166_A_5_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4902/16166_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000122565-CBX3
❮
❯
Specifications
- Immunogen
- Recombinant Protein of HP-1γ
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CBX3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- The antibody was purified from mouse ascites by immunogen affinity chromatography.
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS containing 50% glycerol, pH 7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at -20°C or -80°C. Avoid repeated freeze.
- Shipping
- Ice packs
Applications
- Application Notes
- Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500
Target
- Alternative Names
- Chromobox 3; Heterochromatin Protein 1 Homolog Gamma; Modifier 2 Protein; HECH; Chromobox Homolog 3 (HP1 Gamma Homolog, Drosophila); Chromobox Homolog 3 (Drosophila HP1 Gamma); Heterochromatin Protein HP1 Gamma; HP1 Gamma Homolog (Drosophila);
- Gene ID
- 11335
- UniProt ID
- Q13185
- Long Name
- Chromobox 3
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CBX3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-271 | Human Anti-CBX3 Recombinant Antibody (clone D243); Fab Fragment | IP, ELISA, IP-MS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0496 | Hi-Affi™ Rabbit Anti-CBX3 Recombinant Antibody (clone DS496AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX164 | Mouse Anti-CBX3 Recombinant Antibody (clone 6D8-10D10-5A9) | WB, IHC | Mouse IgG1 |
| VS3-QX165 | Mouse Anti-CBX3 Recombinant Antibody (clone 6F7-6A3-2B10) | WB, IHC | Mouse IgG1 |
| VS3-QX166 | Mouse Anti-CBX3 Recombinant Antibody (clone 9E5-1E3-3A8) | WB, IHC | Mouse IgG1 |
| VS3-QX167 | Mouse Anti-CBX3 Recombinant Antibody (clone 5G10-F7-A12) | WB, IF, IP, IHC | Mouse IgG1 |
| VS3-WK923 | Mouse Anti-CBX3 Recombinant Antibody (VS3-WK923) | WB, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC631 | AbPlus™ Anti-CBX3 Magnetic Beads (VS-0724-YC631) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY325 | Anti-CBX3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1038 | Anti-Human CBX3 Immunohistochemistry Kit | IHC |
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