Mouse Anti-CBX5 Recombinant Antibody (VS3-CJ966) (CAT#: VS3-CJ966)
This product is a mouse antibody that recognizes human, and Monkey CBX5.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16699/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16699/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16699/164_F10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16699/164_F10_1_red_green.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/971_A3_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/971_A3_1_red_green.jpg
Figure 4 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_B_7_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_B_7_5.jpg
Figure 5 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_A_9_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_A_9_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_A_8_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_A_8_5.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000094916-CBX5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000094916-CBX5
Specifications
- Immunogen
- Recombinant protein of HP-1α
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Monkey CBX5
- Species Reactivity
- Human, Monkey
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:300).
Target
- Alternative Names
- HP1; HP1A; HEL25
- Gene ID
- 23468
- UniProt ID
- P45973
- Cellular Localization
- Centromere, Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity).
Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
- Protein Refseq
- NP_001120793.1; NP_001120794.1; NP_036249.1
- Function
- Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CBX5"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-276 | Human Anti-CBX5 Recombinant Antibody (clone D244); Fab Fragment | ELISA, IP-MS, ChIP | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-277 | Human Anti-CBX5 Recombinant Antibody (clone D399) | ELISA, IP-MS | Human IgG1 |
| MRO-0764-CN | Mouse Anti-CBX5 Recombinant Antibody (clone B0-1) | WB, IF | Mouse IgG1 |
| MRO-0765-CN | Rabbit Anti-CBX5 Recombinant Antibody (clone CBACN-283) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-QX168 | Mouse Anti-CBX5 Recombinant Antibody (clone 6F1-10H3-6F5) | IHC | Mouse IgG1 |
| VS3-CJ967 | Rabbit Anti-CBX5 Recombinant Antibody (VS3-CJ967) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0498 | Hi-Affi™ Rabbit Anti-CBX5 Recombinant Antibody (clone DS498AB) | WB, IP, IHC-P, FC, IF | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-CJ966. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.