Mouse Anti-CBX5 Recombinant Antibody (ZG-0441J)
CAT#: ZG-0441J
This product is a mouse antibody that recognizes CBX5.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CBX5 Recombinant Antibody (ZG-0441J) in WB
Western blot detection of HP1 alpha in MCF7,293T,HelaNE,3T3,C6,COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa
ICC
Figure 2 Mouse Anti-CBX5 Recombinant Antibody (ZG-0441J) in ICC
Immunocytochemistry stain of Hela using HP1 alpha mouse mAb (1:300).
IHC
Figure 3 Mouse Anti-CBX5 Recombinant Antibody (ZG-0441J) in IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma with HP1 alpha mouse mAb (3G2-H10-A6, 1:400 diluted ),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrat buffer(pH6.0).
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16699/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16699/164_F10_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/971_A3_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000094916-CBX5
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Specifications
- Immunogen
- Purified recombinant human HP1 alpha protein fragments expressed in
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CBX5
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunocytochemistry.
Target
- Alternative Names
- HP1; HP1A; HEL25; chromobox protein homolog 5; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha; antigen p25; chromobox homolog 5 (HP1 alpha homolog, Drosophila); epididymis luminal protein 25; heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha
- Gene ID
- 23468
- UniProt ID
- P45973
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CBX5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-276 | Human Anti-CBX5 Recombinant Antibody (clone D244); Fab Fragment | ELISA, IP-MS, ChIP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-277 | Human Anti-CBX5 Recombinant Antibody (clone D399) | ELISA, IP-MS | Human IgG1 |
| MRO-0764-CN | Mouse Anti-CBX5 Recombinant Antibody (clone B0-1) | WB, IF | Mouse IgG1 |
| MRO-0765-CN | Rabbit Anti-CBX5 Recombinant Antibody (clone CBACN-283) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MOB-0036F | Mouse Anti-CBX5 Recombinant Antibody (clone 2G2) | WB, IHC-P, IF | Mouse IgG |
| MOB-0037F | Mouse Anti-CBX5 Recombinant Antibody (clone 5E3) | IF, WB, IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0498 | Hi-Affi™ Rabbit Anti-CBX5 Recombinant Antibody (clone DS498AB) | WB, IP, IHC-P, FC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY43 | AbPlus™ Anti-CBX5 Magnetic Beads (D399) | IP-MS, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY326 | Anti-CBX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1041 | Anti-Mouse CBX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1040 | Anti-Human CBX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1042 | Anti-Rat CBX5 Immunohistochemistry Kit | IHC |
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