CytoStream™ Mouse Anti-CCL2 Recombinant Antibody (clone 5D3-F7) (CAT#: VS-0225-XY10)
This monoclonal antibody, targeting CCL2, demonstrates exceptional reactivity with human, rhesus, and cynomolgus primate samples. Derived from a mouse source, it is designed for accurate flow cytometry (FC) analysis, offering valuable insights into CCL2-mediated immune responses. Its versatility across multiple species enhances its utility in comparative immunological studies, supporting detailed exploration of CCL2's role in inflammation and immune modulation.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg
Figure 3 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg
Figure 6 Colon
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg
Figure 7 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg
Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
Specifications
- Immunogen
- Recombinant Human CCL2 protein
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- CCL2
- Species Reactivity
- Human, Rhesus, Cynomolgus
- Clone
- 5D3-F7
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS containing BSA
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
- Alternative Names
- CCL2; C-C motif chemokine 2; Chemokine (C-C motif) ligand 2; MCAF; SCYA2
- Gene ID
- 6347
- UniProt ID
- P13500
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Rheumatoid Arthritis
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-038 | Recombinant Anti-Human CCL2 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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