Rabbit Anti-CCND3 Recombinant Antibody (VS3-CJ865) (CAT#: VS3-CJ865)

• Immunogen
• Recombinant protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse CCND3

• Species Reactivity
• Human, Mouse

• Applications
• WB, ICC, IF, FC

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry.

• Alternative Names
• Cyclin D3

• Gene ID
• 896

• UniProt ID
• P30281

• Sequence Similarities
• Belongs to the cyclin family. Cyclin D subfamily.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Phosphorylation at Thr-283 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex.
  Ubiquitinated by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-283 phosphorylation (PubMed:33854235, PubMed:33854239).
  The DCX(AMBRA1) complex represents the major regulator of CCND3 stability during the G1/S transition (PubMed:33854235, PubMed:33854239).
  Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation (PubMed:22020328).

• Protein Refseq
• NP_001129489.1; NP_001129597.1; NP_001129598.1

• Function
• Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition (PubMed:8114739).
  Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase (PubMed:8114739).
  Hypophosphorylates RB1 in early G1 phase (PubMed:8114739).
  Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:8114739).
  Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed:16782892).
  Shows transcriptional coactivator activity with ATF5 independently of CDK4 (PubMed:15358120).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

Select a product category from the dropdown menu below to view related products.