The antibody 7B11 is specific for CCR3 and has the properties of a true receptor antagonist. 7B11 blockes binding of various radiolabeled chemokines to either CCR3 transfectants, or eosinophils. Pretreatment of eosinophils with this mAb blockes chemotaxis and calcium flux induced by all CCR3 ligands.
Figure 1 Involvement of CCR3 in MAPkinase activation by eotaxin.
Eosinophils were preincubated with blocking monoclonal anti-CCR3 antibodies (7B11) or isotypespecific control antibodies for 30 minutes and stimulated with eotaxin (10-8 mol/L) for 1 minute. Cytosolic extracts underwent Western blotting with antibodies against dual threonine-tyrosine–phosphorylated ERK2 (a-PT/PY-ERK1/2) (A) or dual threoninetyrosine–phosphorylated p38 (a-PT/PY-p38) (B). 7B11 blocked the phosphorylation of both ERK2 and p38.
Kampen, G. T., Stafford, S., Adachi, T., Jinquan, T., Quan, S., Grant, J. A., ... & Alam, R. (2000). Eotaxin induces degranulation and chemotaxis of eosinophils through the activation of ERK2 and p38 mitogen-activated protein kinases: Presented in part at the annual meetings of the American Academy of Allergy, Asthma, and Immunology in Washington, DC, March 13-18, 1998, and in Orlando, FL, February 26-March 3, 1999. Blood, 95(6), 1911-1917.
Figure 2 CCR3 functional assays.
Western blot analysis demonstrating the kinetics of phosphorylation of the p42 mitogen-activated protein kinase in eotaxin-stimulated PBECs. PBECs were preincubated with media alone or pertussis toxin (1 μg/ml, 1 h) and stimulated with eotaxin (100 ng/ml) for up to 1 min. Similar results were noted when lysates were analyzed by immunoblot for eotaxin-induced tyrosine phosphorylation with and without preincubation with the CCR3-blocking mAb 7B11. (These are representative blots of n = 3 for primary epithelial cells and n = 3 for 16-HBE cells.) To ensure equal loading in each lane, we load each lane with 10 μg of total protein (measured by the bicinchoninic acid assay) and run a parallel immunoblot probed with p42/p44 anti-ERK Ab.
Stellato, C., Brummet, M. E., Plitt, J. R., Shahabuddin, S., Baroody, F. M., Liu, M. C., ... & Beck, L. A. (2001). Cutting edge: expression of the CC chemokine receptor CCR3 in human airway epithelial cells. The Journal of Immunology, 166(3), 1457-1461.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1624z | Mouse Anti-CCR3 Recombinant Antibody (clone 27C5) | WB, ELISA, FC | Mouse IgG1 |
MOB-0369CT | Mouse Anti-CCR3 Recombinant Antibody (clone 6F9) | FC, IF, IHC-Fr | Mouse IgG2a |
FAMAB-1029CQ | Mouse Anti-CCR3 Recombinant Antibody (clone 5E8-G9-B4) | FC | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-266CQ | Rat Anti-CCR3 Recombinant Antibody (clone CBL302) | FC, IHC, CyTOF, Neut | Rat IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0521 | Hi-Affi™ Rabbit Anti-CCR3 Recombinant Antibody (clone DS521AB) | WB, IHC-P, ICC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0308-CN-S(P) | Mouse Anti-CCR3 Recombinant Antibody (clone 7B11); scFv Fragment | FC, IF | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0308-CN-F(E) | Mouse Anti-CCR3 Recombinant Antibody (clone 7B11); Fab Fragment | FC, IF | Mouse Fab |
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