Rabbit Anti-CCR3 Recombinant Antibody (clone R82-6D-6) (CAT#: VS3-FY2111)
This is a rabbit recombinant monoclonal antibody, which binds to human CCR3. It cross-reacts with mouse, rat. The R82-6D-6 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69514/1943_C10_3_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69514/1943_C10_3_selected.jpg
Figure 2 Esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_B_6_1.jpg
Figure 3 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_7_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_7_4.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_7_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_4_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_9_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enriched (RPMI-8226)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183625-CCR3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183625-CCR3
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CCR3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R82-6D-6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 41 kDa, while the observed molecular weight stands at 60kDa.
- Related Disease
- Folliculotropic Mycosis Fungoides and Aids Dementia Complex
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- C-C Motif Chemokine Receptor 3; Chemokine (C-C Motif) Receptor 3; Eosinophil Eotaxin Receptor; C-C CKR-3; CC-CKR-3; CMKBR3; CCR-3; CKR3
- Gene ID
- 1232
- UniProt ID
- P51677
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P51677)
Modification sites at neXtProt (NX_P51677)
Glycosylation from GlyGen (P51677) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_006713023.1; XP_016861174.1; XP_054201143.1; XP_054201144.1; NP_001158152.1
- Function
- Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1624z | Mouse Anti-CCR3 Recombinant Antibody (clone 27C5) | WB, ELISA, FC | Mouse IgG1 |
| MOB-0369CT | Mouse Anti-CCR3 Recombinant Antibody (clone 6F9) | FC, IF, IHC-Fr | Mouse IgG2a |
| FAMAB-0308-CN | Mouse Anti-CCR3 Recombinant Antibody (clone 7B11) | FC, IF, WB | Mouse IgG |
| FAMAB-1029CQ | Mouse Anti-CCR3 Recombinant Antibody (clone 5E8-G9-B4) | FC | Mouse IgG2b |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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