Rabbit Anti-CCR3 Recombinant Antibody (clone R82-6D-6)
CAT#: VS3-FY2111
This is a rabbit recombinant monoclonal antibody, which binds to human CCR3. It cross-reacts with mouse, rat. The R82-6D-6 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69514/1943_C10_3_selected.jpg
Normal Tissue
Figure 2 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_B_6_1.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183625-CCR3
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CCR3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R82-6D-6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 41 kDa, while the observed molecular weight stands at 60kDa.
- Related Disease
- Folliculotropic Mycosis Fungoides and Aids Dementia Complex
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- C-C Motif Chemokine Receptor 3; Chemokine (C-C Motif) Receptor 3; Eosinophil Eotaxin Receptor; C-C CKR-3; CC-CKR-3; CMKBR3; CCR-3; CKR3
- Gene ID
- 1232
- UniProt ID
- P51677
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P51677)
Modification sites at neXtProt (NX_P51677)
Glycosylation from GlyGen (P51677) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_006713023.1; XP_016861174.1; XP_054201143.1; XP_054201144.1; NP_001158152.1
- Function
- Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CCR3"
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0724-YC1047 | AbPlus™ Anti-CCR3 Magnetic Beads (VS-0724-YC1047) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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