Human Anti-CCR4 Recombinant Antibody (clone 9E10J)
CAT#: HPAB-1008WJ
This product is a human antibody binds to an epitope in the extracellular domain of human CC chemokine receptor 4 (CCR4) and which are capable of inhibiting the binding of macrophage-derived chemokine (MDC) and/or thymus and activation regulated chemokine (TARC) to CCR4.
Published Data
Gene Expression
FC
Figure 1 Affinity assessment of the improved anti-CCR4 antibodies.
Analysis of dissociation rates in the cell surface retention assay in vitro on DT40/CCR+ cells. Either scFv molecules cross-linked with PE-labeled anti-cMyc antibody (a) or human IgG1 molecules labeled with Alexa Fluor 488 (b) were used for cell binding. In all experiments the unlabeled 9E10J IgG1 molecules were added to the cells to prevent rebinding of labeled molecules; decay of the fluorescence signal was recorded. Values are expressed as a percentage of the initial median fluorescence intensity.
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
Inhib
Figure 2 Affinity assessment of the improved anti-CCR4 antibodies.
Inhibition of labeled ligands, CCL22 (f) or CCL17 (g), binding to DT40/CCR4 cells in the presence of increasing concentrations of unlabeled IgG 9E10J, 306, 406, 503, control antibody KW-0761var and of the corresponding unlabeled ligand.
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
FC
Figure 3 Effect of human anti-CCR4 antibody 9E10J on platelet aggregation.
Binding of 9E10J IgG1 at concentration 10 µg/mL to human platelets isolated from the fresh donor blood. As negative controls, binding histograms for an isotype control antibody (Isotype) and of a secondary antibody alone (anti-human-PE) are shown.
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
FuncS
Figure 4 Effect of human anti-CCR4 antibody 9E10J on platelet aggregation.
Ligand-induced aggregation of platelets pre-incubated with either the isotype control antibody (d) or anti-CCR4 antibody 9E10J (e).
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
Cyt
Figure 5 ADCC activity of anti-CCR4 antibodies using different tumor cell lines and autologous Treg cells as targets.
Comparison of the ADCC activity on T-cell leukemia CCRF-CEM cells of the affinity improved variant 9E10J to its parent antibody 9E (a) and a comparator antibody KM3060var (b).
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/329_F5_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in a subset of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/68832_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/68832_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/68832_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183813-CCR4
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Specifications
- Host Species
- Human
- Type
- Human IgG1, λ
- Specificity
- Human CCR4
- Species Reactivity
- Human
- Clone
- 9E10J
- Applications
- FC, Cyt, Inhib, FuncS, ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CCR4 antibody has been reported in applications of Flow Cytometry, Cytotoxicity,Inhibition, Functional Assay, Enzyme-linked Immunosorbent Assay, Western Blot.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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