Human Anti-CCR4 Recombinant Antibody (clone 9E10J) (CAT#: HPAB-1008WJ)
This product is a human antibody binds to an epitope in the extracellular domain of human CC chemokine receptor 4 (CCR4) and which are capable of inhibiting the binding of macrophage-derived chemokine (MDC) and/or thymus and activation regulated chemokine (TARC) to CCR4.
Specific Inquiry
FC
Figure 1 Affinity assessment of the improved anti-CCR4 antibodies.
Analysis of dissociation rates in the cell surface retention assay in vitro on DT40/CCR+ cells. Either scFv molecules cross-linked with PE-labeled anti-cMyc antibody (a) or human IgG1 molecules labeled with Alexa Fluor 488 (b) were used for cell binding. In all experiments the unlabeled 9E10J IgG1 molecules were added to the cells to prevent rebinding of labeled molecules; decay of the fluorescence signal was recorded. Values are expressed as a percentage of the initial median fluorescence intensity.
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
Inhib
Figure 2 Affinity assessment of the improved anti-CCR4 antibodies.
Inhibition of labeled ligands, CCL22 (f) or CCL17 (g), binding to DT40/CCR4 cells in the presence of increasing concentrations of unlabeled IgG 9E10J, 306, 406, 503, control antibody KW-0761var and of the corresponding unlabeled ligand.
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
FC
Figure 3 Effect of human anti-CCR4 antibody 9E10J on platelet aggregation.
Binding of 9E10J IgG1 at concentration 10 µg/mL to human platelets isolated from the fresh donor blood. As negative controls, binding histograms for an isotype control antibody (Isotype) and of a secondary antibody alone (anti-human-PE) are shown.
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
FuncS
Figure 4 Effect of human anti-CCR4 antibody 9E10J on platelet aggregation.
Ligand-induced aggregation of platelets pre-incubated with either the isotype control antibody (d) or anti-CCR4 antibody 9E10J (e).
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
Cyt
Figure 5 ADCC activity of anti-CCR4 antibodies using different tumor cell lines and autologous Treg cells as targets.
Comparison of the ADCC activity on T-cell leukemia CCRF-CEM cells of the affinity improved variant 9E10J to its parent antibody 9E (a) and a comparator antibody KM3060var (b).
Hagemann, U. B., Gunnarsson, L., Géraudie, S., Scheffler, U., Griep, R. A., Reiersen, H., ... & Kiprijanov, S. M. (2014). Fully human antagonistic antibodies against CCR4 potently inhibit cell signaling and chemotaxis. PLoS One, 9(7), e103776.
Specifications
- Host Species
- Human
- Type
- Human IgG1, λ
- Specificity
- Human CCR4
- Species Reactivity
- Human
- Clone
- 9E10J
- Applications
- FC, Cyt, Inhib, FuncS, ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CCR4 antibody has been reported in applications of Flow Cytometry, Cytotoxicity,Inhibition, Functional Assay, Enzyme-linked Immunosorbent Assay, Western Blot.
Target
- Alternative Names
- C-C Motif Chemokine Receptor 4; Chemokine (C-C Motif) Receptor 4; C-C CKR-4; CC-CKR-4; CMKBR4; CCR-4; K5-5; C-C Chemokine Receptor Type 4;
- Gene ID
- 1233
- UniProt ID
- P51679
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-187 | Human Anti-CCR4 Recombinant Antibody (TAB-187) | Neut, ELISA, IF, IP, FuncS, FC, ICC | Human IgG1, κ |
| TAB-1530CL | Human Anti-CCR4 Recombinant Antibody (TAB-1530CL) | Depletion, FuncS | Human IgG4 |
| TAB-1530CL-S(P) | Human Anti-CCR4 Recombinant Antibody; scFv Fragment (TAB-1530CL-S(P)) | Depletion, FuncS | Human scFv |
| TAB-1530CL-F(E) | Human Anti-CCR4 Recombinant Antibody; Fab Fragment (TAB-1530CL-F(E)) | Depletion, FuncS | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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