Mouse Anti-CD14 Recombinant Antibody (clone 18E12)
CAT#: HPAB-1538WJ
This product is a mouse antibody targeting human CD14. This antibody is useful to treat and prevent disorders with CD14 mediated cell activation, such as sepsis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/1183_E12_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (A549, CACO-2, SiHa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
❮
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Specifications
- Immunogen
- Purified human recombinants CD14
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG1
- Specificity
- Human CD14
- Species Reactivity
- Human
- Clone
- 18E12
- Applications
- ELISA, WB, FC
- Related Disease
- CD14 mediated cell activation
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Customer Review
There are currently no Customer reviews or questions for HPAB-1538WJ. Click the button above to contact us or submit your feedback about this product.
Excellent for Flow Cytometry
This antibody worked really well in our flow cytometry experiments. The specificity was high, and there was minimal background noise. Highly recommend for researchers looking into immune cell profiling
Reliable in Western Blotting
I've used this antibody in various western blot assays, and it consistently produces clear, strong bands. Very dependable for analyzing CD14 expression in human cells.
Clear Results in Western Blot
Very satisfied with how clear and distinct the bands were in our western blot experiments. This is now our go-to antibody for CD14 detection in our studies.
Q&As
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What is the specificity of the Mouse Anti-CD14 Recombinant Antibody (clone 18E12)?
A: The clone 18E12 of the Mouse Anti-CD14 Recombinant Antibody binds to the CD14 molecule, which is a co-receptor for the detection of bacterial lipopolysaccharide (LPS) and is important for the innate immune system.
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What is the species reactivity of the Mouse Anti-CD14 Recombinant Antibody (clone 18E12)?
A: Human CD14 antigen is the predominant species reactivity, guaranteeing its application in human-related immunological studies.
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Are there any known cross-reactivities with other CD antigens?
A: Its strong specificity for CD14 is confirmed by the lack of notable cross-reactivities with other CD antigens.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| HPAB-0101-YC-F(E) | Mouse Anti-CD14 Recombinant Antibody (clone 28C5); Fab Fragment | ELISA, FC, Block | Mouse Fab |
| HPAB-N0031-YC-F(E) | Human Anti-CD14 Recombinant Antibody (clone P1A8); Fab Fragment | IF, WB | Human Fab |
| HPAB-1538WJ-F(E) | Mouse Anti-CD14 Recombinant Antibody; Fab Fragment (clone 18E12) | ELISA, WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0031-YC | Human Anti-CD14 Recombinant Antibody (clone P1A8) | IF, WB | Human IgG |
| ZG-0079J | Mouse Anti-CD14 Recombinant Antibody (ZG-0079J) | IHC-P, IF | Mouse IgG |
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| CAT | Product Name | Application | Type |
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