Rabbit Anti-CD163 Recombinant Antibody (clone 7B2)
CAT#: ZG-0584J
This product is a rabbit antibody that recognizes CD163.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-CD163 Antibody (ZG-0584J) in IHC
IHC image of ZG-0584J diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 2 Rabbit Anti-CD163 Antibody (ZG-0584J) in IHC
IHC image of ZG-0584J diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
FC
Figure 3 Rabbit Anti-CD163 Antibody (ZG-0584J) in FC
Overlay histogram showing Raw264.7 cells stained with ZG-0584J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
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Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46404/1373_A6_3_selected.jpg
Normal Tissue
Figure 2 Lung
Macrophages
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46404/134927_A_3_4.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46404/134927_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46404/134927_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (GAMG, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177575-CD163
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Specifications
- Immunogen
- A synthesized peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD163
- Species Reactivity
- Human
- Clone
- 7B2
- Applications
- ELISA, IHC, FC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
IHC:1:50-1:500
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOB-164-S(P) | Recombinant Anti-human CD163 Antibody scFv Fragment | FC, ELISA, FuncS | scFv |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0724-YC520 | AbPlus™ Anti-CD163 Magnetic Beads (VS-0724-YC520) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-1024-XY81 | Mouse Anti-NHP CD163 Recombinant Antibody (clone EDHu-1) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0325-XY354 | Anti-CD163 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1120 | Anti-Human CD163 Immunohistochemistry Kit | IHC | |
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| CAT | Product Name | Application | Type |
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