Rabbit Anti-CD19 Recombinant Antibody (clone 4C7)

Name: Rabbit Anti-CD19 Recombinant Antibody (clone 4C7)
SKU: ZG-0586J
Rating: 5 (1 reviews)

CAT#: ZG-0586J

This product is a rabbit antibody that recognizes CD19.

Tested Data Gene Expression

Figure 1 Rabbit Anti-CD19 Antibody (ZG-0586J) in WB

Western Blot
Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate
All lanes: CD19 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 95 kDa

IHC

Figure 2 Rabbit Anti-CD19 Antibody (ZG-0586J) in IHC

IHC image of ZG-0586J diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-CD19 Antibody (ZG-0586J) in IP

Immunoprecipitating CD19 in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0586J in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0586J(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)

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Normal Tissue Figure 1 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg

Normal Tissue Figure 2 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg

Normal Tissue Figure 3 Appendix

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19

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Specifications

Immunogen
A synthesized peptide derived from human CD19

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human CD19

Species Reactivity
Human

Clone
4C7

Applications
ELISA, WB, IHC, IP

Conjugate
Uconjugated

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000

Target

Alternative Names
B4; CVID3; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; T-cell surface antigen Leu-12; differentiation antigen CD19

Gene ID
930

UniProt ID
P15391

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0586J.pdf

MSDS

MSDS - ZG-0586J.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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