Mouse Anti-CD19 Recombinant Antibody (clone 8G11B7) (CAT#: ZG-0240U)
Mouse anti-CD19 antibody (clone 8G11B7) displayed high specificity against the CD19 protein of Human.
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WB
Figure 1 Mouse Anti-CD19 Antibody (ZG-0240U) in WB
Positive WB detected in: Raji whole cell lysate All lanes: CD19 antibody at 1: 2000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 95 kDa
IHC
Figure 2 Mouse Anti-CD19 Antibody (ZG-0240U) in IHC
IHC image of ZG-0240U diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Mouse Anti-CD19 Antibody (ZG-0240U) in IF
Immunofluorescence staining of Hela cells with ZG-0240U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 4 Mouse Anti-CD19 Antibody (ZG-0240U) in FC
Overlay histogram showing Raji cells stained with ZG-0240U (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- Recombinant Human CD19 protein (20-291AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CD19
- Species Reactivity
- Human
- Clone
- 8G11B7
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency, Common Variable, 3 and Common Variable Immunodeficiency.
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:2000-1:80000, IHC:1:50-1:200, IF:1:50-1:200
Target
- Alternative Names
- B4; CVID3; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; T-cell surface antigen Leu-12; differentiation antigen CD19
- Gene ID
- 930
- UniProt ID
- P15391
Related Resources
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-174 | Mouse Anti-CD19 Recombinant Antibody (clone Taplitumomab) | ELISA, IP, FC, FuncS, Neut, IF, ICC | Mouse IgG1, κ |
| TAB-1611CL | Mouse Anti-CD19 Recombinant Antibody (TAB-1611CL) | Depletion, FuncS | Mouse IgG2a, κ |
| TAB-1620CL | Anti-Human CD19 Recombinant Antibody (HD37) | FuncS | |
| TAB-1622CL | Mouse Anti-CD19 Recombinant Antibody (TAB-1622CL) | ELISA, FC, Apop, ADCC, FuncS | Mouse IgG |
| TAB-1623CL | Mouse Anti-CD19 Recombinant Antibody (TAB-1623CL) | ELISA, FC | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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