Mouse Anti-CD22 Recombinant Antibody (clone RFB-4)

Name: Mouse Anti-CD22 Recombinant Antibody (clone RFB-4)
SKU: VS3-CJ767
Rating: 5 (1 reviews)

CAT#: VS3-CJ767

This product is a mouse antibody that recognizes human CD22.

Gene Expression

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg

Normal Tissue Figure 2 Spleen

Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22

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Specifications

Immunogen
Native purified human CD22

Host Species
Mouse

Type
Mouse IgG1, κ

Specificity
Human CD22

Species Reactivity
Human

Clone
RFB-4

Applications
IF, FC

Conjugate
Unconjugated

Product Property

Purification
Affinity chromatography

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.3.

Preservative
0.02% Sodium Azide

Storage
Store at 4°C for one year. Do not freeze.

Shipping
Shipped at 4°C

Applications

Application Notes
This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

Target

Alternative Names
SIGLEC2; SIGLEC-2

Gene ID
933

UniProt ID
P20273

Sequence Similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Cellular Localization
Cell membrane, Membrane

Post Translation Modifications
Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN.

Protein Refseq
NP_001172028.1; NP_001172029.1; NP_001172030.1

Function
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-CJ767.pdf

MSDS

MSDS - VS3-CJ767.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

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