CytoStream™ Rat Anti-CD226 Recombinant Antibody (clone 10E5) (CAT#: VS-0225-XY25)
This rat-derived monoclonal antibody targets CD226, a cell surface receptor implicated in immune cell activation. With specificity for mouse CD226, it proves invaluable for flow cytometry (FC) applications, enabling precise analysis of immune cell populations and interactions. The antibody's design ensures robust binding and accurate detection, making it an essential tool in studies related to immune signaling and T cell responses.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15865/1847_C10_42_cr5abb48a309a8f_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15865/1847_C10_42_cr5abb48a309a8f_selected.jpg
Figure 2 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in cells outside reaction centra.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in cells outside reaction centra.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/37861_B_7_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/37861_B_7_5.jpg
Figure 4 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/37861_A_9_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/37861_A_9_8.jpg
Figure 5 RNA cell line category: Group enriched (HEL, HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150637-CD226
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150637-CD226
Specifications
- Host Species
- Rat
- Type
- Rat IgG2b, kappa
- Specificity
- CD226
- Species Reactivity
- Mouse
- Clone
- 10E5
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Blocking Antibody Products
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3326z | Mouse Anti-CD226 Recombinant Antibody (clone 11F4) | WB, IP, FC | Mouse IgG1, κ |
| FAMAB-1574CQ | Mouse Anti-CD226 Recombinant Antibody (clone 10E5) | FC | Mouse IgG1 |
| VS3-FY264 | Recombinant Mouse Anti-CD226 Antibody (clone 4D8E1B4) | ELISA, IHC, FC | Mouse IgG1 |
| VS3-FY265 | Recombinant Mouse Anti-CD226 Antibody (clone 5C3F1C3) | ELISA, IHC, FC | Mouse IgG1 |
| VS3-FY266 | Recombinant Rabbit Anti-Cd226 Antibody (clone R01-4C1) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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