CytoStream™ Rat Anti-CD226 Recombinant Antibody (clone 10E5)
CAT#: VS-0225-XY25
This rat-derived monoclonal antibody targets CD226, a cell surface receptor implicated in immune cell activation. With specificity for mouse CD226, it proves invaluable for flow cytometry (FC) applications, enabling precise analysis of immune cell populations and interactions. The antibody's design ensures robust binding and accurate detection, making it an essential tool in studies related to immune signaling and T cell responses.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15865/1847_C10_42_cr5abb48a309a8f_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in cells outside reaction centra.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/37861_B_7_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15715/37861_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HEL, HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150637-CD226
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Specifications
- Host Species
- Rat
- Type
- Rat IgG2b, kappa
- Specificity
- CD226
- Species Reactivity
- Mouse
- Clone
- 10E5
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 10E5"
- CAT
- Product Name
See other products for "Cd226"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-316CQ | Mouse Anti-CD226 Recombinant Antibody (clone DX11) | FC, FuncS, IP, Block | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-317CQ | Mouse Anti-CD226 Recombinant Antibody (clone CBL052) | WB, Neut | Mouse IgG1 |
| NEUT-318CQ | Mouse Anti-CD226 Recombinant Antibody (clone CBL514) | WB, FC, CyTOF, Neut | Mouse IgG1 |
| NEUT-319CQ | Mouse Anti-CD226 Recombinant Antibody (clone MM0248-1X20) | WB, Neut, IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4034 | Hi-Affi™ Rabbit Anti-CD226 Recombinant Antibody (clone SI100DS) | ELISA | Rabbit IgG |
| MOR-4035 | Hi-Affi™ Rabbit Anti-CD226 Recombinant Antibody (clone SI101DS) | ELISA | Rabbit IgG |
| MOR-4036 | Hi-Affi™ Rabbit Anti-CD226 Recombinant Antibody (clone SI102DS) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-097CQ | Rat Anti-CD226 Recombinant Antibody (MOB-097CQ) | FC, FuncS | Rat IgG2b, κ |
| FN-153CQ | Rat Anti-CD226 Recombinant Antibody (clone CBL-1316) | FC, FuncS, Neut, IP | Rat IgG2b, κ |
| VS3-FY264 | Recombinant Mouse Anti-CD226 Antibody (clone 4D8E1B4) | ELISA, IHC, FC | Mouse IgG1 |
| VS3-FY265 | Recombinant Mouse Anti-CD226 Antibody (clone 5C3F1C3) | ELISA, IHC, FC | Mouse IgG1 |
| VS3-FY266 | Recombinant Rabbit Anti-Cd226 Antibody (clone R01-4C1) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC693 | Recombinant Anti-CD226 Vesicular Antibody, EV Displayed (VS-0425-YC693) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1144 | Anti-CD226 Immunohistochemistry Kit | IHC |
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