Anti-CD274 (clone W2410F.9G2) Recombinant Antibody Coupled Liposome (VS-1024-FY42)
CAT#: VS-1024-FY42
PD-L1-targeted immunoliposomes (P-Lipo) represent a groundbreaking approach in cancer therapy, designed to selectively deliver therapeutic agents to tumor cells expressing the PD-L1 protein. By promoting immunogenic cell death (ICD), these immunoliposomes not only enhance the visibility of tumor cells to the immune system but also facilitate a more robust and sustained anti-tumor immune response. The synergy achieved through the combination of P-Lipo and conventional immunotherapies has the potential to significantly improve treatment outcomes, offering hope for more effective management of various cancers.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
Normal Tissue
Figure 5 Placenta
Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
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More Infomation
Specifications
- Potential Clinical Applications
- Immunogenic cell death (ICD) effect, antitumor immunity
- Immunoliposomes Method
- The preparation method for antibody liposomes (P-Lipo) is as follows:
1. Complex Formation: First, form the IRI-PC complex.
2. Film Preparation: Mix the complex with a chloroform solution containing lecithin, cholesterol, DSPE-PEG2000, and DSPE-PEG2000-NHS in a weight ratio of 20/2/1/0.05. Subsequently, perform rotary evaporation to create a thin film.
3. Film Hydration: Hydrate the thin film using a 5% glucose solution, followed by ultrasound treatment in a water bath.
4. Extrusion: Subject the preparation to extrusion through polycarbonate membranes with pore sizes of 400 nm and 200 nm to obtain uniform liposomes.
5. Removal of Unencapsulated Drug: Utilize a Sephadex G-50 column to remove any unencapsulated drug.
6. Antibody Conjugation: Add anti-PD-L1 antibody to the liposomes, maintaining a molar ratio of antibody to DSPE-PEG2000-NHS of 1:100, and allow the reaction to proceed for 12 hours.
7. Particle Size Analysis: Measure the particle size distribution of the liposomes using dynamic light scattering technology (Nano ZS 90).
Product Composition
- Clone
- W2410F.9G2
- Antibody Type
- IgG2b, kappa
- Antibody Host
- Rat
- Antibody Reactivity
- Mouse
- Antibody Description
- The monoclonal antibody W2410F.9G2 specifically targets mouse PD-L1 (programmed death ligand 1), also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that is part of the B7 family within the immunoglobulin superfamily. This protein is expressed on various immune and non-immune cells, including T lymphocytes, B lymphocytes, natural killer (NK) cells, dendritic cells, as well as monocytes stimulated by interferon-gamma, epithelial cells, and endothelial cells. The W2410F.9G2 antibody has been demonstrated to inhibit the binding of PD-L1 to both PD-1 and B7-1 (CD80).
Product Property
- Storage
- See in the COA
- Storage Shelf Time
- See in the COA
Target Information
- Target
- CD274
- Alternative Names
- CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1
- Full Name
- CD274 Molecule
- Gene ID
- 29126
- UniProt ID
- Q9NZQ7
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. BTN/MOG family.
- Cellular Localization
- Cell membrane, Endosome, Membrane, Nucleus, Secreted
- Post Translation Modifications
- Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation (PubMed:28813410).
Ubiquitinated by MARCHF8; leading to degradation (PubMed:34183449).
Glycosylation at Asn35, Asn192, Asn200, and Asn219 (NX_Q9NZQ7 [NX_Q9NZQ7-1])
Modification sites at PhosphoSitePlus (Q9NZQ7)
Glycosylation from GlyGen (Q9NZQ7) 5 sites
- Protein Refseq
- XP_047279218.1; XP_054218785.1; NP_001254635.1; NP_001300958.1; NP_054862.1
- Function
- Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410).
As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response.
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
- CAT
- Product Name
See other products for "Cd274"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1814z | Mouse Anti-CD274 Recombinant Antibody (clone 7A5) | FC, IHC, FuncS | Mouse IgG2b |
| HPAB-0196-YC | Human Anti-CD274 Recombinant Antibody (HPAB-0196-YC) | FC | Human IgG |
| HPAB-0197-YC | Rabbit Anti-CD274 Recombinant Antibody (HPAB-0197-YC) | ELISA, FC | Rabbit IgG |
| HPAB-0198-YC | Mouse Anti-CD274 Recombinant Antibody (HPAB-0198-YC) | ELISA, FC, Block | Mouse IgG |
| HPAB-0199-YC | Human Anti-CD274 Recombinant Antibody (HPAB-0199-YC) | ELISA, FC, Block | Chimeric (mouse/human) IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0190CL | Anti-Human CD274 Recombinant Antibody (PD1-AB1) | FC, ELISA, FuncS | |
| TAB-0997CL-S(P) | Anti-Human CD274 Recombinant Antibody scFv Fragment (B60-55) | ELISA | Human antibody |
| TAB-1002CL-S(P) | Mouse Anti-CD274 Recombinant Antibody (TAB-1002CL-S(P)); scFv Fragment | ELISA, FC | Mouse scFv |
| TAB-1003CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-1003CL-S(P)) | FC, ELISA | Human scFv |
| TAB-1004CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-1004CL-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0262CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0262CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Mouse IgG |
| TAB-0992CL-S(P) | Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0992CL-S(P)) | FC | Mouse scFv |
| TAB-0993CL-S(P) | Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0993CL-S(P)) | FC | Mouse scFv |
| TAB-0994CL-S(P) | Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0994CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0262CL-F(E) | Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0262CL-F(E)) | ELISA, WB, SPR, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0995CL | Human Anti-CD274 Recombinant Antibody (TAB-0995CL) | ELISA, FC | Chimeric (Mouse/Human) IgG1, κ |
| TAB-0995CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0995CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0263CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0263CL-F(E)) | ELISA, WB, SPR, FuncS | Humanized Fab |
| TAB-0264CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0264CL-F(E)) | ELISA, WB, SPR, Inhib, FuncS | Humanized Fab |
| TAB-0265CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0265CL-F(E)) | ELISA, WB, SPR, FuncS | Humanized Fab |
| TAB-0266CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0266CL-F(E)) | ELISA, WB, SPR, FuncS | Humanized Fab |
| TAB-0267CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0267CL-F(E)) | ELISA, WB, SPR, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-324CQ | Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6) | IHC-P, FC, Neut, ICC | Mouse IgG2b, κ |
| NEUT-325CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-206) | Neut | Human IgG1, κ |
| NEUT-326CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-207) | ELISA, Neut | Human IgG1, κ |
| NEUT-327CQ | Mouse Anti-CD274 Recombinant Antibody (clone MIH1) | FC, FuncS, Neut, IHC, IF | Mouse IgG1, κ |
| NEUT-330CQ | Rat Anti-CD274 Recombinant Antibody (clone MIH5) | FC, FuncS, Neut, IHC-Fr, WB | Rat IgG2a, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-328CQ | Rat Anti-CD274 Recombinant Antibody (VMC171) | Block, FC | Rat IgG2 |
| NEUT-329CQ | Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2) | FC, IF, Block | Rat IgG2b, κ |
| NEUT-331CQ | Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1) | ELISA, Block | Mouse IgG1, λ |
| NEUT-332CQ | Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009) | Block | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0548 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB) | ELISA | Rabbit IgG |
| MOR-4049 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS) | ELISA | Rabbit IgG |
| MOR-4050 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS) | ICC, IF, FC | Rabbit IgG |
| MOR-4051 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1440LY-S(P) | Rabbit Anti-CD274 Recombinant Antibody scFv Fragment (HPAB-1440LY-S(P)) | IHC | Rabbit scFv |
| HPAB-0400LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0400LY-S(P)) | ELISA, RIA, WB | Human scfv |
| HPAB-0401LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0401LY-S(P)) | ELISA, RIA, WB | Human scfv |
| HPAB-0402LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0402LY-S(P)) | ELISA, RIA, WB | Human scfv |
| HPAB-0403LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0403LY-S(P)) | ELISA, RIA, WB | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0561LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0561LY-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
| HPAB-0562LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0562LY-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
| HPAB-0563LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0563LY-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
| HPAB-0564LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0564LY-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
| HPAB-0565LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0565LY-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-417CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| AFC-TAB-418CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ) | ELISA, IHC, FC, IP, IF, BL | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NBD-0065-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH) | ELISA | Camelid VHH |
| NBD-0066-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH) | ELISA, Inihb | Camelid VHH |
| NBD-0067-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0067-YJ-VHH) | ELISA, FC | Camelid VHH |
| NBD-0068-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0068-YJ-VHH) | ELISA, FC | Camelid VHH |
| NBD-0069-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0069-YJ-VHH) | ELISA, FC | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC110 | Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced | ELISA, WB, SPR, ADCC, CDC | Antibody hexamer |
| VS-0924-YC111 | Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced | ELISA, WB, SPR, ADCC, CDC, Inhib | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY375 | Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY32 | CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY65 | Human Anti-CD274 (clone MI4736) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC608 | Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608) | Block, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1152 | Anti-CD274 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1153 | Anti-Mouse CD274 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1154 | Anti-Human CD274 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC202 | Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv | ELISA, IHC, FC, IP, IF | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC8 | SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC8) | ELISA | Mouse IgG |
| VS-0825-YC47 | SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC47) | ELISA, IHC, FC, IP, IF, Inhib | Human IgG1 kappa |
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