Human Anti-CD3 Recombinant Antibody (clone HuM291) (CAT#: HPAB-0546-WJ)

Figure 1 Antiglobulin cross-linking of HuM291-IgG2M3 inhibits apoptosis.

After preactivation for 96 h by a solid phase-bound anti-CD3 mAb, BC3, T cells were harvested, and re-exposed for 20 h to soluble OKT3 or HuM291-IgG2M3 with or without antihuman IgG2 in the presence of 50 U/ml IL-2. Cell samples were stained with PI and were acquired on a FACScan at a constant flow rate for 45 s. Cell death was determined by averaging the number of viable (PI-negative) cells remaining in triplicate samples.

Carpenter, P. A., Pavlovic, S., Tso, J. Y., Press, O. W., Gooley, T., Yu, X. Z., & Anasetti, C. (2000). Non-Fc receptor-binding humanized anti-CD3 antibodies induce apoptosis of activated human T cells. The Journal of Immunology, 165(11), 6205-6213.

Figure 2 Soluble anti-CD3 mAbs induce apoptosis in activated human peripheral human T cells.

Preactivated T cells were exposed to HuM291-IgG2M3 and then stained with FITC-conjugated annexin V and PI at the time points indicated (bottom). Flow cytometry was used to determine the percentage of cells in early apoptosis (annexin V positive and PI negative), late apoptosis (annexin V and PI positive), and viable cells (annexin V and PI negative).

Carpenter, P. A., Pavlovic, S., Tso, J. Y., Press, O. W., Gooley, T., Yu, X. Z., & Anasetti, C. (2000). Non-Fc receptor-binding humanized anti-CD3 antibodies induce apoptosis of activated human T cells. The Journal of Immunology, 165(11), 6205-6213.