Mouse Anti-CD300LF Recombinant Antibody (VS3-FY2654)
CAT#: VS3-FY2654
The anti-CD300LF antibody is a mouse recombinant monoclonal antibody, which has been intricately crafted to target the CD300F protein, a calculated weight of 32.3 kDa. It shows capability of cross-reacting with human. This monoclonal antibody can be used in Immunohistochemistry, Flow cytometry, ELISA.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117003_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_5_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117003_B_6_4.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186074-CD300LF
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- CD300 protein like family member F of human
- Species Reactivity
- Human
- Applications
- IHC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 32.3 kDa.
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- CD300 Molecule Like Family Member F; Immune Receptor Expressed On Myeloid Cells 1; Immunoglobulin Superfamily Member 13; CD300 Antigen-Like Family Member F; NK Inhibitory Receptor; CD300f; IREM-1; IgSF13; CLM-1; IREM1
- Gene ID
- 146722
- Sequence Similarities
- Belongs to the CD300 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated on tyrosine.
Glycosylation at Asn88
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 5 sites, 4 O-linked glycans (4 sites)
- Protein Refseq
- XP_016879701.1; XP_047291363.1; XP_047291364.1; XP_047291365.1; XP_047291366.1
- Function
- Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731).
Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells.
Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CD300LF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2406MZ | Mouse Anti-CD300LF Recombinant Antibody (clone NFN-370) | FC, WB | Mouse IgG1 |
| HPAB-N0327-YC | Mouse Anti-CD300LF Recombinant Antibody (HPAB-N0327-YC) | ELISA, FC, WB | Mouse IgG1, κ |
| HPAB-1039-FY | Human Anti-CD300LF Recombinant Antibody (clone 871.1) | FC | Chimeric (mouse/human) IgG |
| HPAB-1040-FY | Human Anti-CD300LF Recombinant Antibody (clone B11) | FC | Chimeric (mouse/human) IgG |
| HPAB-1041-FY | Human Anti-CD300LF Recombinant Antibody (clone D12) | FC | Chimeric (mouse/human) IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0327-YC-S(P) | Mouse Anti-CD300LF Recombinant Antibody; scFv Fragment (HPAB-N0327-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
| HPAB-1039-FY-F(E) | Mouse Anti-CD300LF Recombinant Antibody (clone 871.1); scFv Fragment | FC | Mouse scFv |
| HPAB-1040-FY-F(E) | Mouse Anti-CD300LF Recombinant Antibody (clone B11); scFv Fragment | FC | Mouse scFv |
| HPAB-1041-FY-F(E) | Mouse Anti-CD300LF Recombinant Antibody (clone D12); scFv Fragment | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0327-YC-F(E) | Mouse Anti-CD300LF Recombinant Antibody; Fab Fragment (HPAB-N0327-YC-F(E)) | ELISA, FC, WB | Mouse Fab |
| HPAB-1039-FY-S(P) | Human Anti-CD300LF Recombinant Antibody (clone 871.1); Fab Fragment | FC | Chimeric (mouse/human) Fab |
| HPAB-1040-FY-S(P) | Human Anti-CD300LF Recombinant Antibody (clone B11); Fab Fragment | FC | Chimeric (mouse/human) Fab |
| HPAB-1041-FY-S(P) | Human Anti-CD300LF Recombinant Antibody (clone D12); Fab Fragment | FC | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC451 | Recombinant Anti-CD300LF Vesicular Antibody, EV Displayed (VS-0425-YC451) | ELISA, FC, Cell-uptake |
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