Mouse Anti-CD300LF Recombinant Antibody (VS3-FY2654) (CAT#: VS3-FY2654)
The anti-CD300LF antibody is a mouse recombinant monoclonal antibody, which has been intricately crafted to target the CD300F protein, a calculated weight of 32.3 kDa. It shows capability of cross-reacting with human. This monoclonal antibody can be used in Immunohistochemistry, Flow cytometry, ELISA.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/ihc_selected.jpg
(Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117003_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117003_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_5_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_5_6.jpg
Figure 6 Bone marrow
(Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117003_B_6_4.jpg
(Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117003_B_6_4.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13712/117230_A_7_8.jpg
Figure 8 RNA cell line category: Group enriched (THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186074-CD300LF
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186074-CD300LF
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- CD300 protein like family member F of human
- Species Reactivity
- Human
- Applications
- IHC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 32.3 kDa.
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- CD300 Molecule Like Family Member F; Immune Receptor Expressed On Myeloid Cells 1; Immunoglobulin Superfamily Member 13; CD300 Antigen-Like Family Member F; NK Inhibitory Receptor; CD300f; IREM-1; IgSF13; CLM-1; IREM1
- Gene ID
- 146722
- Sequence Similarities
- Belongs to the CD300 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated on tyrosine.
Glycosylation at Asn88
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 5 sites, 4 O-linked glycans (4 sites)
- Protein Refseq
- XP_016879701.1; XP_047291363.1; XP_047291364.1; XP_047291365.1; XP_047291366.1
- Function
- Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731).
Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells.
Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2406MZ | Mouse Anti-CD300LF Recombinant Antibody (clone NFN-370) | FC, WB | Mouse IgG1 |
| HPAB-N0327-YC | Mouse Anti-CD300LF Recombinant Antibody (HPAB-N0327-YC) | ELISA, FC, WB | Mouse IgG1, κ |
| HPAB-1039-FY | Human Anti-CD300LF Recombinant Antibody (clone 871.1) | FC | Chimeric (mouse/human) IgG |
| HPAB-1040-FY | Human Anti-CD300LF Recombinant Antibody (clone B11) | FC | Chimeric (mouse/human) IgG |
| HPAB-1041-FY | Human Anti-CD300LF Recombinant Antibody (clone D12) | FC | Chimeric (mouse/human) IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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