The antibody is the Fab fragment antibody of IgG against CD4 molecule. The CD4 antigen acts as a coreceptor in conjunction with the T cell receptor on T lymphocytes to recognize antigens displayed by antigen-presenting cells in the context of class II MHC molecules.
Figure 1 Mouse Anti-CD4 Recombinant Antibody Fab Fragment in SDS-PAGE
SDS-PAGE analysis of FAMAB-0054-YC-F(E) in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrated as about 25-35 kDa. And non-reduced protein migrated about 48 kDa.
Figure 2 Mouse Anti-CD4 Recombinant Antibody Fab Fragment in SEC-HPLC
The purity of FAMAB-0054-YC-F(E) was 99.4046% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Mouse Anti-CD4 Recombinant Antibody Fab Fragment in WB
Western blot analysis of FAMAB-0054-YC-F(E) was performed by loading human CD4 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with FAMAB-0054-YC-F(E) and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 4 Mouse Anti-CD4 Recombinant Antibody Fab Fragment in DB
Dot Blot analysis of FAMAB-0054-YC-F(E) was performed by coating with human CD4 protein (His tag).
FAMAB-0054-YC-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 5 Mouse Anti-CD4 Recombinant Antibody Fab Fragment in ELISA
ELISA analysis of FAMAB-0054-YC-F(E) was performed by coating with human CD4 protein (His tag). Then blocked with BSA and incubated with FAMAB-0054-YC-F(E). The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-039 | Anti-Human CD4 Recombinant Antibody (Zanolimumab) | Neut, ELISA, IF, IP, FuncS, FC, IHC | IgG1 - kappa |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-107 | Anti-Human CD4 Recombinant Antibody (Cedelizumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG4 - kappa |
TAB-161LC-F(E) | Anti-Human CD4 Recombinant Antibody Fab Fragment (gOKT4A-4) | ELISA | Humanized antibody |
TAB-168LC-F(E) | Anti-Human CD4 Recombinant Antibody Fab Fragment (TRX1) | ELISA | Humanized antibody |
TAB-171LC-F(E) | Human Anti-CD4 Recombinant Antibody; Fab Fragment (TAB-171LC-F(E)) | ELISA, SPR | Humanized Fab |
TAB-172LC-F(E) | Human Anti-CD4 Recombinant Antibody; Fab Fragment (TAB-172LC-F(E)) | ELISA | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G078E | Anti-CD4 immunotoxin M-T151 (IgG)-PE | Cytotoxicity assay, Function study | |
AGTO-G078R | Anti-CD4 immunotoxin M-T151 (IgG)-RTA | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-426 | Human Anti-CD4 Recombinant Antibody (clone 576) | WB, ELISA, FuncS | Human IgG |
PABL-427 | Human Anti-CD4 Recombinant Antibody (clone Hu5A8) | ELISA, Cyt, WB, Neut | Human IgG4 |
HPAB-0135CQ | Human Anti-CD4 Recombinant Antibody (HPAB-0135CQ) | ELISA, Inhib, FuncS | Human IgG1, κ |
HPAB-0136CQ | Mouse Anti-CD4 Recombinant Antibody (HPAB-0136CQ) | ELISA, FuncS | Mouse IgG1, κ |
HPAB-0137CQ | Mouse Anti-CD4 Recombinant Antibody (clone RIB 5/2) | IHC, FC, FuncS | Mouse IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-423 | Human Anti-CD4 Recombinant Antibody (clone 576); scFv Fragment | WB, ELISA, FuncS | Human scFv |
PSBL-424 | Human Anti-CD4 Recombinant Antibody (clone Hu5A8); scFv Fragment | Block, Neut | Human scFv |
HPAB-0557-WJ-S(P) | Human Anti-CD4 Recombinant Antibody (clone TRX1); scFv Fragment | ELISA | Human scFv |
HPAB-0558-WJ-S(P) | Human Anti-CD4 Recombinant Antibody (clone 6G5); scFv Fragment | ELISA | Human scFv |
HPAB-0957WJ-S(P) | Human Anti-CD4 Recombinant Antibody (clone 4-91); scFv Fragment | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-162LC-S(P) | Anti-Human CD4 Recombinant Antibody scFv Fragment | ELISA | Chimeric antibody (mouse/human) |
TAB-164LC-S(P) | Monkey Anti-CD4 Recombinant Antibody; scFv Fragment (TAB-164LC-S(P)) | FC, SPR, ELISA | Monkey scFv |
TAB-165LC-S(P) | Monkey Anti-CD4 Recombinant Antibody; scFv Fragment (TAB-165LC-S(P)) | FC, SPR, ELISA | Monkey scFv |
TAB-167LC-S(P) | Anti-Human CD4 Recombinant Antibody scFv Fragment (cM-T412) | ELISA | Chimeric antibody (mouse/human) |
TAB-170LC-S(P) | Rat Anti-CD4 Recombinant Antibody; scFv Fragment (TAB-170LC-S(P)) | ELISA, SPR | Rat scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-160LC-F(E) | Anti-Human CD4 Recombinant Antibody Fab Fragment (OKT4A) | ELISA | |
TAB-166LC-F(E) | Anti-Human CD4 Recombinant Antibody Fab Fragment (M-T412) | ELISA | |
TAB-169LC-F(E) | Rat Anti-CD4 Recombinant Antibody; Fab Fragment (TAB-169LC-F(E)) | ELISA, SPR | Rat Fab |
TAB-173LC-F(E) | Anti-Human CD4 Recombinant Antibody Fab Fragment (IgA F425-Alg8) | ELISA | |
TAB-174LC-F(E) | Mouse Anti-CD4 Recombinant Antibody; Fab Fragment (TAB-174LC-F(E)) | ELISA, FC, IH | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-025LC | Recombinant Anti-Human CD4 Antibody (Fab glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-351CQ | Mouse Anti-CD4 Recombinant Antibody (clone 10C12) | ELISA, WB, IF, Block | Mouse IgG1 |
NEUT-352CQ | Mouse Anti-CD4 Recombinant Antibody (clone 13B8.2) | Block, ELISA, FC, IHC-Fr, WB | Mouse IgG1, κ |
NEUT-355CQ | Mouse Anti-CD4 Recombinant Antibody (clone RPA-T4) | FC, CyTOF, Block, IHC | Mouse IgG1, κ |
NEUT-357CQ | Rat Anti-CD4 Recombinant Antibody (clone MAB0810) | Block, Depletion, Stim | Rat IgG2b |
NEUT-358CQ | Rat Anti-CD4 Recombinant Antibody (clone RM4-5) | FC, CyTOF, Depletion, Block, IHC | Rat IgG2a, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-353CQ | Mouse Anti-CD4 Recombinant Antibody (clone CBL976) | FC, Neut, IF, IHC | Mouse IgG1, κ |
NEUT-354CQ | Recombinant Mouse Anti-CD4 Antibody (CT-4) | Neut, FC, IHC, IHC-Fr, IHC-P, IP | IgG1, κ |
NEUT-356CQ | Mouse Anti-CD4 Recombinant Antibody (clone 3-4F4) | Neut, FC, IHC, IHC-Fr, IP, ICC | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0560 | Hi-Affi™ Rabbit Anti-CD4 Recombinant Antibody (clone DS560AB) | IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0758-CN-F(E) | Human Anti-CD4 Recombinant Antibody (clone Variant B); Fab Fragment | ELISA, FC | Human Fab |
HPAB-0759-CN-F(E) | Human Anti-CD4 Recombinant Antibody (clone Variant D); Fab Fragment | ELISA, FC | Human Fab |
HPAB-0792-CN-F(E) | Mouse Anti-CD4 Recombinant Antibody (clone B4); Fab Fragment | ELISA | Mouse Fab |
HPAB-0793-CN-F(E) | Human Anti-CD4 Recombinant Antibody (clone chB4); Fab Fragment | ELISA | Chimeric (mouse/human) Fab |
HPAB-0794-CN-F(E) | Human Anti-CD4 Recombinant Antibody (clone dB4C7); Fab Fragment | ELISA | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-039 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Zanolimumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-164 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Priliximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-260 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Tregalizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-171 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Keliximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
AFC-TAB-107 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Cedelizumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
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