Rabbit Anti-CD44 Antibody (VS-0924-YC28) - Cancer Stem Cell Marker (CAT#: VS-0924-YC28)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human CD44

• Species Reactivity
• Human

• Applications
• IHC, WB, IF

• Clonality
• Polyclonal

• Purification
• Affinity purified using the PrEST antigen as affinity ligand

• Concentration
• 0.3 mg/mL

• Buffer
• PBS, pH 7.2, with 40% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry (IHC): 1/1000-1/2500
  Western Blot (WB): 0.1-0.5 µg/mL
  Immunofluorescence (IF): 0.5-2 µg/mL

• Alternative Names
• CD44 Molecule (Indian Blood Group); Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein 1; Hyaluronate Receptor; Hermes Antigen; CD44 Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3

• Gene ID
• 960

• UniProt ID
• P16070

• Long Name
• CD44 molecule (Indian blood group)

• Cellular Localization
• Cell membrane, Cell projection, Membrane

• Post Translation Modifications
• Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
  N-glycosylated.
  O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
  Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

• Protein Refseq
• NP_000601.3; NP_001001389.1; NP_001001390.1

• Function
• Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066).
  Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188).
  Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287).
  Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

• MOB-0264MC
• Goat Anti-Rabbit IgG Antibody (HRP)

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