Rat Anti-CD44 Recombinant Antibody (clone 9A4) (CAT#: HPAB-N0328-YC)

Figure 1 Expression of CD44v6 (9A4 antibody) and ICAM-1 (11C81 antibody) in 3LL cells or hepatocytes derived from Cd44+/+ (WT) or Cd44−/− (KO) mice measured by Western blot analysis.

Olaku, V., Matzke, A., Mitchell, C., Hasenauer, S., Sakkaravarthi, A., Pace, G.,... & Orian-Rousseau, V. (2011). c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice. Molecular biology of the cell, 22(15), 2777-2786.

Figure 2 HGF binding of HT29 cells or HepG2 cells measured by FC analysis.

Gray area, staining with secondary antibody (without HGF). Gray line, binding of HGF. Dotted line, binding of HGF in presence of ICAM-1 antibody. Black line, binding of HGF in presence of CD44v6 antibody.

Olaku, V., Matzke, A., Mitchell, C., Hasenauer, S., Sakkaravarthi, A., Pace, G.,... & Orian-Rousseau, V. (2011). c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice. Molecular biology of the cell, 22(15), 2777-2786.

Figure 3 HGF-induced Erk phosphorylation in BSp73AS cells transiently transfected (Amaxa) with expression vectors for ICAM-1 or CD44v6 or an empty vector as indicated. Bottom, the expression of ICAM-1 and CD44v6 (1.1ASML antibody) monitored in Western blots.

Olaku, V., Matzke, A., Mitchell, C., Hasenauer, S., Sakkaravarthi, A., Pace, G.,... & Orian-Rousseau, V. (2011). c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice. Molecular biology of the cell, 22(15), 2777-2786.

Figure 4 CD44 expression on LB cells recovered from local growth, remote axillary lymph node 1 spleen and cell culture.

Surface marker expression on LB cells directly isolated from lymphoid organs and local growth was examined by indirect immunofluorescence followed by flow cytometry. Left histogram: incubation with secondary antibody alone. Right histogram: incubation with specific and secondary antibodies. Similar results were obtained in 6 separate experiments.

Vogt Sionov and, R., & Naor, D. (1997). Hyaluronan‐independent lodgment of CD44+ lymphoma cells in lymphoid organs. International journal of cancer, 71(3), 462-469.

Figure 5 The expression of CD44 (IM7), CD44v6 (9A4) and smooth muscle actin (SMA) in prepubertal (2 week old) and pubertal (7 week old) mouse mammary glands.

(A) Stained ducts from 2-week-old mice. (B) Stained ducts from 7-week-old mice. For each antibody, ducts are shown with no CD44 staining (upper duct in each left panel), CD44 staining restricted to the myoepithelium (central inset panel in each case) and CD44 staining in both myoepithelial and luminal epithelial layers (right panel in each case). (C) Stained ducts from 7-weekold mice showing CD44 expression in the terminal portions of mammary ducts. Transverse section through TEBs (upper right panel in each case) and terminal portions of ducts (upper left panel in each case) are shown. The lower panel for each antibody shows a longitudinal section through the terminal portion of a mammary duct. The arrows denote areas of ductal epithelium where CD44 expression can be seen in both the myoepithelial layer and the luminal layer. Note that while all of the myoepithelium is positive for CD44 expression, not all areas of the duct show CD44 expression in the luminal cells. Bar, 50 µm.

Hebbard, L., Steffen, A., Zawadzki, V., Fieber, C., Howells, N., Moll, J.,... & Sleeman, J. (2000). CD44 expression and regulation during mammary gland development and function. J Cell Sci, 113(14), 2619-2630.

Figure 6 The expression of CD44 and SMA in the mammary gland during pregnancy, lactation and involution. Positive staining is brown-red in color. The sections were counterstained with hematoxylin.

(A) CD44v6 staining in early pregnancy. Left panel: SMA. Right panel: 9A4. (B) CD44 staining during lactation. Left panel: SMA. Central panel: 9A4 (left and central panels are serial sections from mammary glands 10 days into lactation). Right panel: IM7. (C) SMA and 9A4 staining of large collecting ducts 12 days into lactation (left and central panels, respectively). Right panel: CD44v6 expression during involution of the mammary gland 8 days after weaning. Bar, 50 µm.

Hebbard, L., Steffen, A., Zawadzki, V., Fieber, C., Howells, N., Moll, J.,... & Sleeman, J. (2000). CD44 expression and regulation during mammary gland development and function. J Cell Sci, 113(14), 2619-2630.