CytoStream™ Rat Anti-CD44 Recombinant Antibody (clone KM114) (CAT#: VS-0225-XY54)
A highly versatile antibody targeting CD44, this reagent exhibits strong reactivity with mouse cells, supporting a broad range of applications including flow cytometry, immunohistochemistry, western blot, ELISA, and blocking assays. Its exceptional specificity and performance make it an essential tool for studies of cell adhesion, migration, and signaling pathways. This antibody provides reliable results across various experimental platforms, ensuring accuracy in immune and cancer research.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg
Figure 3 IHC staining of human vagina
(Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg
(Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg
Figure 4 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg
Figure 6 Skin
(Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg
(Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
Specifications
- Host Species
- Rat
- Type
- Rat IgG1, kappa
- Specificity
- CD44
- Species Reactivity
- Mouse
- Clone
- KM114
- Applications
- FC, IHC, WB, Block, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD44"
Select a product category from the dropdown menu below to view related products.
Please select product type
Humanized Antibody Products
Mouse Antibody Products
Human Antibody Products
Chimeric Antibody Products
Chicken IgY Antibody Products
Blocking Antibody Products
Neutralizing Antibody Products
Rabbit Monoclonal Antibody Products
Fab Antibody Products
ScFv Antibody Products
IgG Antibody Products
IgM Antibody Products
ADCC Enhanced Antibody Products
Antibody Magnetic Beads
Cancer Stem Cell Biomarker Specific Antibody Products
Non-Human Primate (NHP) Antibody Products
ScFv-Fc Chimera Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-727 | Anti-CD44 Recombinant Antibody (Bivatuzumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 |
| TAB-236LC | Anti-Human CD44 Recombinant Antibody (huPTA-4621) | FuncS | Humanized antibody |
| TAB-236LC-S(P) | Anti-Human CD44 Recombinant Antibody scFv Fragment (huPTA-4621) | FC | Humanized antibody |
| TAB-236LC-F(E) | Anti-Human CD44 Recombinant Antibody Fab Fragment (huPTA-4621) | FC | Humanized antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS-0225-XY54. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
-1.jpg)
Application: IB, ELISA, FC, FuncS
Application: WB, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC
-1.png)
Application: WB, ELISA, FuncS
Application: ELISA, Inhib, FuncS
Application: WB, IHC-Fr, IHC-P, ICC, IF
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
