Human Anti-CD47 Recombinant Antibody (HPAB-0008-FY) (CAT#: HPAB-0008-FY)

Figure 1 Anti-Human CD47 Antibody in WB

Western blot analysis of HPAB-0008-FY was performed by loading Recombinant Human CD47 Protein (lane 1, non-reducing antigen: 1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0008-FY and Goat Anti-Human IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.

Figure 2 Anti-Human CD47 Antibody in ELISA

ELISA analysis of HPAB-0008-FY was performed by coating with Recombinant Human CD47 Protein. Then blocked with BSA, and incubated with Anti-Human CD47 Antibody (HPAB-0008-FY). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 3 Anti-Human CD47 Antibody (HPAB-0008-FY) in SDS-PAGE

SDS-PAGE analysis of HPAB-0008-FY in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 4 Anti-Human CD47 Antibody (HPAB-0008-FY) in HPLC

The purity of this antibody is> 95% as determined by SEC-HPLC.