This product is a neutralizing human CD47 antibody derived from hybridomas. antibody used in various therapeutic approaches, CD47 is a ubiquitously expressed transmembrane glycoprotein with a single Ig-like domain and five transmembrane regions that serves as a cellular ligand for SIRPa through the NH2-terminal V-like structure of SIRPa Domain mediates binding.
Figure 1 Anti-Human CD47 Antibody in WB
Western blot analysis of HPAB-0008-FY was performed by loading Recombinant Human CD47 Protein (lane 1, non-reducing antigen: 1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0008-FY and Goat Anti-Human IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.
Figure 2 Anti-Human CD47 Antibody in ELISA
ELISA analysis of HPAB-0008-FY was performed by coating with Recombinant Human CD47 Protein. Then blocked with BSA, and incubated with Anti-Human CD47 Antibody (HPAB-0008-FY). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Human CD47 Antibody (HPAB-0008-FY) in SDS-PAGE
SDS-PAGE analysis of HPAB-0008-FY in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 4 Anti-Human CD47 Antibody (HPAB-0008-FY) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-278LC | Human Anti-CD47 Recombinant Antibody (TAB-278LC) | Block, FuncS | Human IgG, κ |
TAB-279LC | Human Anti-CD47 Recombinant Antibody (TAB-279LC) | Block, FuncS | Human IgG, κ |
TAB-278LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-278LC-S(P)) | ELISA | Human scFv |
TAB-278LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-278LC-F(E)) | ELISA | Human Fab |
TAB-279LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-279LC-F(E)) | ELISA | Human Fab |
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0008-FY, RRID: AB_3111342)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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