Human Anti-CD47 Recombinant Antibody (HPAB-0008-FY) (CAT#: HPAB-0008-FY)
This product is a neutralizing human CD47 antibody derived from hybridomas. antibody used in various therapeutic approaches, CD47 is a ubiquitously expressed transmembrane glycoprotein with a single Ig-like domain and five transmembrane regions that serves as a cellular ligand for SIRPa through the NH2-terminal V-like structure of SIRPa Domain mediates binding.
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WB
Figure 1 Anti-Human CD47 Antibody in WB
Western blot analysis of HPAB-0008-FY was performed by loading Recombinant Human CD47 Protein (lane 1, non-reducing antigen: 1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0008-FY and Goat Anti-Human IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human CD47 Antibody in ELISA
ELISA analysis of HPAB-0008-FY was performed by coating with Recombinant Human CD47 Protein. Then blocked with BSA, and incubated with Anti-Human CD47 Antibody (HPAB-0008-FY). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
SDS-PAGE
Figure 3 Anti-Human CD47 Antibody (HPAB-0008-FY) in SDS-PAGE
SDS-PAGE analysis of HPAB-0008-FY in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
HPLC
Figure 4 Anti-Human CD47 Antibody (HPAB-0008-FY) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
Specifications
- Immunogen
- Hu5F9-G4 recognizes human and cynomolgus CD47, but not mouse CD47.
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human CD47
- Species Reactivity
- Human, Cynomolgus monkey
- Applications
- ELISA, PD, Activ, PK, Stim
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD47 Molecule; Antigenic Surface Determinant Protein OA3; CD47 Antigen (Rh-Related Antigen, Integrin-Associated Signal Transducer); Antigen Identified By Monoclonal Antibody 1D8; Leukocyte Surface Antigen CD47; Integrin Associated Protein; Rh-Related Antigen; CD47 Glycoprotein
- Gene ID
- 961
- UniProt ID
- Q08722
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-278LC | Human Anti-CD47 Recombinant Antibody (TAB-278LC) | Block, FuncS | Human IgG, κ |
| TAB-279LC | Human Anti-CD47 Recombinant Antibody (TAB-279LC) | Block, FuncS | Human IgG, κ |
| TAB-278LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-278LC-S(P)) | ELISA | Human scFv |
| TAB-279LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-279LC-S(P)) | ELISA | Human scFv |
| TAB-278LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-278LC-F(E)) | ELISA | Human Fab |
Customer Reviews and Q&As
Customer Review
Q&As
User-friendly
User-friendly design and clear instructions make this antibody product easy to incorporate into our existing protocols without any hassle.
High sensitivity
The high sensitivity of this antibody product has enabled us to detect even low-abundance targets with confidence.
Reliable shipping
Shipping has always been reliable, with the product arriving in perfect condition and on time, which is crucial for our time-sensitive research.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0008-FY, RRID: AB_3111342)
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For Research Use Only. Not For Clinical Use.
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