Rabbit Anti-CD7 Recombinant Antibody (clone R03-8G3)
CAT#: VS3-FY1677
Clone R03-8G3 is a rabbit recombinant monoclonal antibody that is designed to detect human CD7. We recommend antibody dilution: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). Western blot demonstrated that R03-8G3 recognized a target protein with a molecular weight of 25 kDa.
Gene Expression
Normal Tissue
Figure 1 Colon
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39079/133943_A_8_3.jpg
Normal Tissue
Figure 2 Spleen
Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39079/133943_B_7_4.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (Hep G2, JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173762-CD7
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Specifications
- Immunogen
- Synthetic peptide of human CD7
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD7
- Species Reactivity
- Human
- Clone
- R03-8G3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 25 kDa, while the observed molecular weight stands at 35-40 kDa.
- Related Disease
- Pityriasis Lichenoides Et Varioliformis Acuta and T-Cell Leukemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- CD7 Molecule; T-Cell Leukemia Antigen; T-Cell Surface Antigen Leu-9; T-Cell Antigen CD7; CD7 Antigen (P41); P41 Protein
- Gene ID
- 924
- UniProt ID
- P09564
- Cellular Localization
- Membrane
- Protein Refseq
- NP_006128.1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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