Mouse Anti-CD74 Recombinant Antibody (clone In-1)
CAT#: FAMAB-1683CQ
This product is a recombinant mouse antibody that recognizes CD74. The antibody was purified by affinity chromatography. It can be used in IP, FC, IHC-Fr.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10592/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79994/170659_A_6_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/203_F11_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000019582-CD74
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Specifications
- Immunogen
- Mouse lymphocytes of strain A injected into Wistar rats.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Mouse CD74
- Species Reactivity
- Mouse
- Clone
- In-1
- Applications
- IP, FC, IHC-Fr
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- II; p33; DHLAG; HLADG; Ia-GAMMA
- UniProt ID
- CD74
REVIEWS AND Q&AS
CITATIONS
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Solid Conjugation Essentials
Used this antibody with different fluorescent dyes, and the conjugation step went smoothly. The results in FACS were clear and specific.
Smooth Handling Experience
Handling this antibody has been straightforward. Aliquotting and thawing on ice have worked perfectly according to the provided protocols.
Sturdy Even at Room Temperature
Accidently left the antibody at room temperature for a couple of hours, and it still performed well. Shows good stability.
Q&As
-
Can I aliquot the antibody for several applications?
A: Indeed, aliquoting the antibody into smaller amounts can help shield it from deteriorating as a result of repeated freeze-thaw cycles.
-
What is the ideal pH for antibody storage?
A: Antibodies are typically stable at physiological pH ranges of 7.2-7.4. For exact recommendations, consult the product information page.
-
Do I have to filter the antibody solution?
A: If the solution is visibly turbid, or if the manufacturer's procedure calls for it, filtration might be necessary. Use a filter with a low capacity for protein binding to minimize antibody loss.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone In-1"
- CAT
- Product Name
See other products for "CD74"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0004CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0004CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| TAB-0005CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0005CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| TAB-0006CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0006CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| TAB-0007CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0007CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| TAB-0008CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0008CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0569CL-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (TAB-0569CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0570CL-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (TAB-0570CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0571CL-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (TAB-0571CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0572CL-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (TAB-0572CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0573CL-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (TAB-0573CL-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0454MZ | Recombinant Mouse Anti-Human CD74 Antibody (clone MO3 (Xpsltipq JW) (pvs eftjhobujpo 349)) | ICC, IHC-Fr, IHC-P, WB | Mouse antibody |
| MRO-0316-CN | Rabbit Anti-CD74 Recombinant Antibody (clone JF40-10) | WB, IF, IHC, FC | Rabbit IgG |
| FAMAB-1081CQ | Mouse Anti-CD74 Recombinant Antibody (clone YAML 555.6) | ELISA | Mouse IgG1 |
| VS3-QX199 | Mouse Anti-CD74 Recombinant Antibody (clone 1267CT820.116.140.154) | WB, FC | Mouse IgG2b |
| VS3-FY316 | Recombinant Mouse Anti-CD74 Antibody (clone 1B7-6A7-5G10) | IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-408CQ | Mouse Anti-CD74 Recombinant Antibody (clone LN2) | FC, IHC-Fr, IHC-P, ICC, IP, WB, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0579 | Hi-Affi™ Rabbit Anti-CD74 Recombinant Antibody (clone DS579AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (HPAB-0388-FY-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY-F(E) | Human Anti-CD74 Recombinant Antibody; Fab Fragment (HPAB-0388-FY-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY55 | AbPlus™ Anti-CD74 Magnetic Beads (4ALN2) | IP, Protein Purification | |
| VS-0724-YC321 | AbPlus™ Anti-CD74 Magnetic Beads (VS-0724-YC321) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY110 | Mouse Anti-NHP CD74 Recombinant Antibody (clone LN-2) | WB, IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY71 | CytoStream™ Mouse Anti-CD74 Recombinant Antibody (clone LN2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY83 | Human Anti-CD74 (clone LL1) scFv-Fc Chimera | FC, ELISA, Cyt | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC330 | Recombinant Anti-CD74 Vesicular Antibody, EV Displayed (VS-0425-YC330) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1217 | Anti-CD74 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1218 | Anti-Mouse CD74 Immunohistochemistry Kit | IHC |
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