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Mouse Anti-CD74 Recombinant Antibody (clone In-1) (CAT#: FAMAB-1683CQ)

This product is a recombinant mouse antibody that recognizes CD74. The antibody was purified by affinity chromatography. It can be used in IP, FC, IHC-Fr.

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  • Conjugation:
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  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/if_selected.jpg
Figure 2 IHC staining of human spleen
Figure 2 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10592/ihc_selected.jpg
Figure 3 IHC staining of human tonsil
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79994/170659_A_6_8_selected.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/203_F11_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Figure 5 Colon
(Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_7_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_5.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_8.jpg
Figure 8 Tonsil
Figure 8 Tonsil
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000019582-CD74

Specifications

  • Immunogen
  • Mouse lymphocytes of strain A injected into Wistar rats.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Specificity
  • Mouse CD74
  • Species Reactivity
  • Mouse
  • Clone
  • In-1
  • Applications
  • IP, FC, IHC-Fr

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • II; p33; DHLAG; HLADG; Ia-GAMMA

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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CAT Product Name Application Type
TAB-0004CL-VHH Anti-Human CD74 VHH Single Domain Antibody (TAB-0004CL-VHH) ELISA, FC, Internalization, Inhib, FuncS VHH
TAB-0005CL-VHH Anti-Human CD74 VHH Single Domain Antibody (TAB-0005CL-VHH) ELISA, FC, Internalization, Inhib, FuncS VHH
TAB-0006CL-VHH Anti-Human CD74 VHH Single Domain Antibody (TAB-0006CL-VHH) ELISA, FC, Internalization, Inhib, FuncS VHH
TAB-0007CL-VHH Anti-Human CD74 VHH Single Domain Antibody (TAB-0007CL-VHH) ELISA, FC, Internalization, Inhib, FuncS VHH
TAB-0008CL-VHH Anti-Human CD74 VHH Single Domain Antibody (TAB-0008CL-VHH) ELISA, FC, Internalization, Inhib, FuncS VHH

Customer Reviews and Q&As

Customer Review Q&As

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Solid Conjugation Essentials
Used this antibody with different fluorescent dyes, and the conjugation step went smoothly. The results in FACS were clear and specific.

Smooth Handling Experience
Handling this antibody has been straightforward. Aliquotting and thawing on ice have worked perfectly according to the provided protocols.

Sturdy Even at Room Temperature
Accidently left the antibody at room temperature for a couple of hours, and it still performed well. Shows good stability.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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