Mouse Anti-CD81 Recombinant Antibody (clone 1.3.3.22)
CAT#: VS3-CJ1059
This product is a mouse antibody that recognizes human CD81.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7234/10_B9_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2507/6520_A_6_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2507/6520_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2507/6520_A_5_6.jpg
Normal Tissue
Figure 5 Endometrium
Cells in endometrial stroma
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2507/6520_B_3_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC diff, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110651-CD81
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Specifications
- Immunogen
- Native purified human CD81
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human CD81
- Species Reactivity
- Human
- Clone
- 1.3.3.22
- Applications
- IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for one year. Do not freeze.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Flow Cytometry.
Target
- Alternative Names
- S5.7; CVID6; TAPA1; TSPAN28
- Gene ID
- 975
- UniProt ID
- P60033
- Sequence Similarities
- Belongs to the tetraspanin (TM4SF) family.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Not glycosylated.
Likely constitutively palmitoylated at low levels. Protein palmitoylation is up-regulated upon coligation of BCR and CD9-C2R-CD81 complexes in lipid rafts.
- Protein Refseq
- NP_001284578.1; NP_004347.1
- Function
- Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells (PubMed:20237408, PubMed:27881302, PubMed:16449649).
Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production (PubMed:15161911, PubMed:20237408).
In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype (PubMed:22307619, PubMed:23858057, PubMed:8766544).
Present in MHC class II compartments, may also play a role in antigen presentation (PubMed:8409388, PubMed:8766544).
Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. Positively regulates sperm-egg fusion and may be involved in acrosome reaction (By similarity).
In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity).
In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480).
Also prevents the fusion of mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity).
May regulate the compartmentalization of enzymatic activities. In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels (PubMed:28871089).
Also involved in cell adhesion and motility. Positively regulates integrin-mediated adhesion of macrophages, particularly relevant for the inflammatory response in the lung (By similarity).
(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Association with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.
(Microbial infection) Involved in SAMHD1-dependent restriction of HIV-1 replication. May support early replication of both R5- and X4-tropic HIV-1 viruses in T cells, likely via proteasome-dependent degradation of SAMHD1.
(Microbial infection) Specifically required for Plasmodium falciparum infectivity of hepatocytes, controlling sporozoite entry into hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "CD81"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-036 | Mouse Anti-CD81 Recombinant Antibody (clone K04) | WB, Block, FuncS | Mouse IgG |
| PABL-428 | Mouse Anti-CD81 Recombinant Antibody (clone K13) | ELISA, WB, FuncS | Mouse IgG |
| MOB-1539MZ | Recombinant Mouse Anti-Human CD81 Antibody (clone N49), PE-Conjugated | FC, FuncS, ICC, IHC-P, IP, WB | Mouse antibody |
| FN-188CQ | Hamster Anti-Cd81 Recombinant Antibody (clone Eat-2 (EAT2)) | FC, FuncS, IP, WB | Hamster IgG |
| ZG-0596J | Rabbit Anti-CD81 Recombinant Antibody (clone 9F7) | ELISA, WB, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-036 | Mouse Anti-CD81 Recombinant Antibody (clone K04); scFv Fragment | WB, Block, FuncS | Mouse scFv |
| PSBL-425 | Mouse Anti-CD81 Recombinant Antibody (clone K13); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| PSBC-257 | Mouse Anti-CD81 Recombinant Antibody (clone Ab4); scFv Fragment | ELISA, FC | Mouse scFv |
| PSBC-258 | Mouse Anti-CD81 Recombinant Antibody (clone Ab5); scFv Fragment | ELISA, FC | Mouse scFv |
| HPAB-0908-YJ-S(P) | Mouse Anti-CD81 Recombinant Antibody; scFv Fragment (HPAB-0908-YJ-S(P)) | ELISA, FC, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-425 | Mouse Anti-CD81 Recombinant Antibody (clone K13); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| PFBC-257 | Mouse Anti-CD81 Recombinant Antibody (clone Ab4); Fab Fragment | ELISA, FC | Mouse Fab |
| PFBC-258 | Mouse Anti-CD81 Recombinant Antibody (clone Ab5); Fab Fragment | ELISA, FC | Mouse Fab |
| PFBC-259 | Mouse Anti-CD81 Recombinant Antibody (clone Ab10); Fab Fragment | ELISA, FC | Mouse Fab |
| HPAB-0908-YJ-F(E) | Mouse Anti-CD81 Recombinant Antibody; Fab Fragment (HPAB-0908-YJ-F(E)) | ELISA, FC, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-348LC | Human Anti-CD81 Recombinant Antibody (TAB-348LC) | ELISA | Human IgG4 |
| TAB-349LC | Human Anti-CD81 Recombinant Antibody (TAB-349LC) | ELISA | Human IgG4 |
| TAB-348LC-S(P) | Human Anti-CD81 Recombinant Antibody; scFv Fragment (TAB-348LC-S(P)) | ELISA | Human scFv |
| TAB-349LC-S(P) | Human Anti-CD81 Recombinant Antibody; scFv Fragment (TAB-349LC-S(P)) | ELISA | Human scFv |
| TAB-348LC-F(E) | Human Anti-CD81 Recombinant Antibody; Fab Fragment (TAB-348LC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-417CQ | Hamster Anti-CD81 Recombinant Antibody (clone 2F7) | Neut, FC, IHC, IHC-Fr, IP, ICC | Hamster IgG3, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-418CQ | Hamster Anti-Cd81 Recombinant Antibody (clone YC081), Biotin | FC, IHC-Fr, IP, Block | Hamster IgG3, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0584 | Hi-Affi™ Rabbit Anti-CD81 Recombinant Antibody (clone DS584AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY74 | CytoStream™ Mouse Anti-CD81 Recombinant Antibody (VS-0225-XY74) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC426 | Recombinant Anti-CD81 Vesicular Antibody, EV Displayed (VS-0425-YC426) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1228 | Anti-CD81 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1229 | Anti-Mouse CD81 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1230 | Anti-Rat CD81 Immunohistochemistry Kit | IHC |
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