Rabbit Anti-CD8A Recombinant Antibody (clone N21-G), FITC
CAT#: ZG-0429U
Rabbit anti-CD8A antibody (clone N21-G) was conjugated with FITC under optimal conditions. The FITC-conjugated antibody product displayed high specificity against the CD8A protein of Human. And the solution is free of unconjugated FITC and unconjugated antibody.
Tested Data
Gene Expression
FC
Figure 1 Rabbit Anti-CD8A Antibody (ZG-0429U) in FC
Profile of human whole blood analyzed by the BD FACSCanto II. Blood cells of healthy control were stained with anti-CD8 FITC (blue; used 10 μl per test).
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1373_A3_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1492_D3_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1377_A3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12/97_A_7_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153563-CD8A
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD8A
- Species Reactivity
- Human
- Clone
- N21-G
- Applications
- FC
- Conjugate
- FITC
- Related Disease
- Cd8 Deficiency, Familial and Herpes Zoster.
Product Property
- Buffer
- Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C, in the dark. Do not freeze.
Applications
- Application Notes
- This antibody has been used 10 µL per test for Flow Cytometry. Specific conditions should be optimized by the end user.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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