CytoStream™ Mouse Anti-CD8A Recombinant Antibody (VS-0225-XY78)
CAT#: VS-0225-XY78
Targeting CD8A, this antibody is highly reactive with human, rhesus, cynomolgus, and baboon species, making it an invaluable tool for cross-species immunological studies. Specifically designed for Flow Cytometry (FC), it enables precise analysis of CD8+ T cells, facilitating in-depth research on cellular immune responses across diverse primate models. This antibody offers exceptional versatility for investigating immune interactions and is an essential reagent for advanced flow cytometric assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1373_A3_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1492_D3_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1377_A3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12/97_A_7_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153563-CD8A
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Specifications
- Immunogen
- Normal Human Thymocytes
- Host Species
- Mouse
- Type
- Mouse IgG2a, kappa
- Specificity
- CD8A
- Species Reactivity
- Human, Rhesus, Cynomolgus, Baboon
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
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- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-0188-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody; scFv Fragment (HPAB-0188-CN-S(P)) | FC | Mouse scFv |
| HPAB-0189-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody; scFv Fragment (HPAB-0189-CN-S(P)) | FC | Mouse scFv |
| FAMAB-0009-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody (clone CT8); scFv Fragment | FC | Mouse scFv |
| FAMAB-0010-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody (clone EP72); scFv Fragment | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-428CQ | Mouse Anti-CD8A Recombinant Antibody (clone UCHT4) | FC, IHC-Fr, IHC-P, IP, Separation, Block | Mouse IgG2a, κ |
| NEUT-431CQ | Rat Anti-CD8A Recombinant Antibody (clone 53-6.7) | ICC, IF, FC, IHC-Fr, IP, FuncS, Block | Rat IgG2a, κ |
| NEUT-432CQ | Rat Anti-CD8A Recombinant Antibody (clone MAB0811) | Block, Depletion | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-429CQ | Mouse Anti-CD8A Recombinant Antibody (clone CT-8) | Neut, FC, IHC, IHC-Fr, IHC-P, IP, ICC | Mouse IgG1, κ |
| NEUT-430CQ | Mouse Anti-CD8A Recombinant Antibody (clone LT8) | WB, Neut | Mouse IgG1 |
| NEUT-433CQ | Mouse Anti-CD8A Recombinant Antibody (clone 76-2-11) | Neut, FC, IHC-Fr, IHC-P, IP | Mouse IgG2a, κ |
| NEUT-434CQ | Mouse Anti-Cd8a Recombinant Antibody (clone OX-8) | WB, Neut, FC, IHC, IHC-Fr, IHC-P, IP, CyTOF® | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0582 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS582AB) | IHC | Rabbit IgG |
| MOR-0589 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS589AB) | IHC-P | Rabbit IgG |
| MOR-0590 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS590AB), PE | FC | Rabbit IgG |
| MOR-0057-FY | Rabbit Anti-CD8A Recombinant Antibody (clone AFY0028) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0041-CN | Mouse Anti-CD8A Recombinant Antibody (clone 13C5) | WB, IP, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0317-CN | Rabbit Anti-CD8A Recombinant Antibody (clone CBACN-121) | WB, IF, IHC | Rabbit IgG |
| FAMAB-1677CQ | Rat Anti-CD8A Recombinant Antibody (clone YCATE55.9) | Block, FC, IF, IHC-Fr | Rat IgG1 |
| FAMAB-1679CQ | Mouse Anti-CD8A Recombinant Antibody (clone YTS 169.4) | Depletion, FuncS, FC, IHC, IHC-Fr | Mouse IgG2a |
| VS3-FY325 | Recombinant Mouse Anti-CD8a Antibody (clone 7H4B10) | ELISA, FC | Mouse IgG1 |
| VS3-FY326 | Recombinant Rabbit Anti-CD8A Antibody (clone R09-8E7) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY114 | Mouse Anti-NHP CD8A Recombinant Antibody (clone T8/209) | ELISA | Mouse IgG1, kappa |
| VS-1024-XY112 | Rat Anti-NHP CD8A Recombinant Antibody (clone YTC182.20) | FC | Rat IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY79 | CytoStream™ Mouse Anti-CD8A Recombinant Antibody (clone SK1) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY375 | Anti-CD8A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1238 | Anti-Mouse CD8A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1237 | Anti-Human CD8A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1240 | Anti-Rat CD8A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC377 | Recombinant Anti-CD8A Vesicular Antibody, EV Displayed (VS-0425-YC377) | ELISA, FC, Neut, Cell-uptake |
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