Mouse Anti-CD9 Recombinant Antibody (clone 5A6D8) (CAT#: ZG-0249U)

Mouse anti-CD9 antibody (clone 5A6D8) displayed high specificity against the CD9 protein of Human.


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WB

Figure 1 Mouse Anti-CD9 Antibody (ZG-0249U) in WB

Figure 1 Mouse Anti-CD9 Antibody (ZG-0249U) in WB

Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate. MG-63 whole cell lysate
All lanes CD9 antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time: 5min

IHC

Figure 2 Mouse Anti-CD9 Antibody (ZG-0249U) in IHC

Figure 2 Mouse Anti-CD9 Antibody (ZG-0249U) in IHC

IHC image of ZG-0249U diluted at 1:50 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • Recombinant Human CD9 antigen protein (112-195AA)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Specificity
  • Human CD9
  • Species Reactivity
  • Human
  • Clone
  • 5A6D8
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Diphtheria and Human Immunodeficiency Virus Infectious Disease

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
    WB:1:2000-1:16000, IHC: 1:50-1:200

Target

  • Alternative Names
  • MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29; CD9 antigen; 5H9 antigen; BA-2/p24 antigen; CD9 antigen (p24); antigen CD9; cell growth-inhibiting gene 2 protein; leukocyte antigen MIC3; motility related protein-1; tetraspanin-29

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CD9"

Blocking Antibody

CAT Product Name Application Type
NEUT-436CQ Mouse Anti-CD9 Recombinant Antibody (clone MM2/57) Block, FC, IHC-Fr, WB Mouse IgG2b
NEUT-437CQ Mouse Anti-CD9 Recombinant Antibody (clone MEM-61) WB, IHC-P, FC, Block, FuncS Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0592 Hi-Affi™ Rabbit Anti-CD9 Recombinant Antibody (clone DS592AB) WB, IP, IHC-P, ICC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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