Mouse Anti-CD9 Recombinant Antibody (clone 5A6D8)
CAT#: ZG-0249U
Mouse anti-CD9 antibody (clone 5A6D8) displayed high specificity against the CD9 protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CD9 Antibody (ZG-0249U) in WB
Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate. MG-63 whole cell lysate
All lanes CD9 antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time: 5min
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/if_selected.jpg
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Specifications
- Immunogen
- Recombinant Human CD9 antigen protein (112-195AA)
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human CD9
- Species Reactivity
- Human
- Clone
- 5A6D8
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Diphtheria and Human Immunodeficiency Virus Infectious Disease
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:2000-1:16000, IHC: 1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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