Mouse Anti-CD9 Recombinant Antibody (clone HI9a) (CAT#: VS3-CJ781)

• Immunogen
• Native purified human CD9

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human CD9

• Species Reactivity
• Human

• Clone
• HI9a

• Applications
• IF, FC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS, pH 7.3.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for one year. Do not freeze.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

• Alternative Names
• MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29

• Gene ID
• 928

• UniProt ID
• P21926

• Sequence Similarities
• Belongs to the tetraspanin (TM4SF) family.

• Cellular Localization
• Cell membrane, Membrane, Secreted

• Post Translation Modifications
• Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids (PubMed:11959120).
  Palmitoylation by ZDHHC2 regulates CD9 expression, association with other tetraspanin family proteins and function in cell adhesion (PubMed:18508921).

• Protein Refseq
• NP_001317241.1; NP_001760.1

• Function
• Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:8478605, PubMed:14575715, PubMed:18541721).
  Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity).
  In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity).
  In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480).
  Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity).
  Acts as a receptor for PSG17 (By similarity).
  Involved in platelet activation and aggregation (PubMed:18541721).
  Regulates paranodal junction formation (By similarity).
  Involved in cell adhesion, cell motility and tumor metastasis (PubMed:8478605, PubMed:7511626).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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